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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 173 - 181 (9page)
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초록· 키워드
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We identified Na+-K+-2Cl- cotransporter 2 (NKCC2) cDNA isoform from starry flounder Platichthys stellate. The NKCC2 cDNA encoded a polypeptide of 1,043 amino acids representing 12 putative transmembrane domains based on the bioinformatic topology prediction. In addition, starry flounder NKCC2 possessed highly conserved residues within the transmembrane domain 4, known as an essential site for its functionality. End-point RT-PCR analysis revealed that NKCC2 transcript was only found in anterior and posterior intestines and rectum, with the moderate expression levels. In response to Streptococcus parauberis challenge, the NKCC2 mRNA level in the rectum, but not in other segments, was significantly induced at 3 day post challenge, indicating that excess salt may be transported into the rectum. Taken together, our data indicate that S. parauberis infection would tip the intestinal fluid balance in favor of fluid accumulation, indicating that the bacterial pathogens can interfere with osmotic balance in the intestine as well as normal mucosal immune homeostasis.
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참고문헌 (33)
참고문헌 신청
Guffey S / 2011 / Regulation of apical H⁺-ATPase activity and intestinal HCO3⁻ secretion in marine fish osmoregulation. Am J Physiol 301, R1682-R1691 / Am J Physiol 301:R1682~R1691
Payne JA / 1994 / Alternative spliced isoforms of putative renal Na-K-Cl cotransporter are differentially distributed within the rabbit kidney / Proc Natl Acad Sci USA 91:4544~4548
Sattin G / 2010 / Cytosolic carbonic anhydrase in the Gulf toadfish is important for tolerance to hypersalinity / Comp Biochem Physiol A 156:169~175
Schmittgen TD / 2008 / Analyzing real-time PCR data by the comparative CT method / Nat Protoc 3:1101~1108
Sundh H / 2014 / Development of intestinal ion‐transporting mechanisms during smoltification and seawater acclimation in Atlantic salmon Salmo salar / J Fish Biol 85:1227~1252
Payne JA / 1994 / Alternative spliced isoforms of putative renal Na-K-Cl cotransporter are differentially distributed within the rabbit kidney / Proc Natl Acad Sci USA 91:4544~4548
Sattin G / 2010 / Cytosolic carbonic anhydrase in the Gulf toadfish is important for tolerance to hypersalinity / Comp Biochem Physiol A 156:169~175
Schmittgen TD / 2008 / Analyzing real-time PCR data by the comparative CT method / Nat Protoc 3:1101~1108
Sundh H / 2014 / Development of intestinal ion‐transporting mechanisms during smoltification and seawater acclimation in Atlantic salmon Salmo salar / J Fish Biol 85:1227~1252
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