지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 339 - 344 (6page)
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초록· 키워드
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PCR amplification with universal primer is a useful tool for speciation of symbionts in marine eukaryote coupled with robust separation method such as denaturing high performance chromatography (DHPLC). To overcome the biased amplification, clamping PCR is recommended to suppress the amplification of host gene. In this study, we evaluated the efficiency of rare gene detection for two kinds of clamping probes which were successfully utilized for eukaryotic symbiont analysis: C3 linked nucleotide (C3) and peptide nucleic acid (PNA). PNA was 3-4 orders of magnitude higher than that of C3 tested in clamping efficiency and rare gene detection. This represented that PNA could be a more competent clamping probe for the enhancement of PCR amplification for rare symbiont genes.
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등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Amann R / 2000 / Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology / FEMS Microbiol Rev 24 : 555 ~ 565
Baker GC / 2003 / Review and re-analysis of domain-specific 16S primers / J Microbiol Meth 55 : 541 ~ 555
Bass D / 2004 / Phylum-specific environmental DNA analysis reveals remarkably high global biodiversity of Cercozoa (Protozoa) / Int J Syst Evol Microbiol 54 : 2393 ~ 2404
Blankenship LE / 2005 / Universal primers and PCR of gut contents to study marine invertebrate diets / Mol Ecol 14 : 891 ~ 899
Bottger EC / 1989 / Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA / FEMS Microbiol Lett 65 : 171 ~ 176
Baker GC / 2003 / Review and re-analysis of domain-specific 16S primers / J Microbiol Meth 55 : 541 ~ 555
Bass D / 2004 / Phylum-specific environmental DNA analysis reveals remarkably high global biodiversity of Cercozoa (Protozoa) / Int J Syst Evol Microbiol 54 : 2393 ~ 2404
Blankenship LE / 2005 / Universal primers and PCR of gut contents to study marine invertebrate diets / Mol Ecol 14 : 891 ~ 899
Bottger EC / 1989 / Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA / FEMS Microbiol Lett 65 : 171 ~ 176
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