지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 273 - 277 (5page)
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초록· 키워드
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Vibriosis caused by Vibrio anguillarum and edwardsiellosis caused by Edwardsiella tarda are septicemic diseases of many commercially important freshwater and marine fishes, and threaten the aquaculture industry in Korea. Early diagnosis and accurateidentification of these two bacterial species could help to prevent these diseases and minimize the damage to cultured marine species. This study designed a duplex polymerase chain reaction (PCR) method for the simultaneous detection of two major fishpathogens: V. anguillarum and E. tarda. Each pair of oligonucleotide primers exclusively amplified the target groEL gene of the specific microorganism. Twenty-two Vibrio and ten non-Vibrio enteric species were used to check the specificity of the primers,which were found to be highly specific for the target species, even among closely related species. The detection limit was 400 pg for V. anguillarum and 4 ng for E. tarda when mixed purified DNA was used as the template. This assay showed high specificity and sensitivity in the simultaneous detection of V. anguillarum and E. tarda from artificially inoculated seawater and fish.
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참고문헌 (22)
참고문헌 신청
Ausubel FM / 1998 / Current Protocols in Molecular Biology / John Wiley and Sons
Castro N / 2010 / Evaluation of four polymerase chain reaction primer pairs for the detection of Edwardsiella tarda in turbot / Dis Aquat Org 90 : 55 ~ 61
Chizhikov V / 2001 / Microarray analysis of microbial virulence factors / Appl Environ Microbiol 67 : 3258 ~ 3263
Frans I / 2011 / Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention / J Fish Dis 34 : 643 ~ 661
Gonzalez SF / 2004 / Simultaneous detection of marine fish pathogens by using multiplex PCR and a DNA microarray / J Clin Microbiol 42 : 1414 ~ 1419
Castro N / 2010 / Evaluation of four polymerase chain reaction primer pairs for the detection of Edwardsiella tarda in turbot / Dis Aquat Org 90 : 55 ~ 61
Chizhikov V / 2001 / Microarray analysis of microbial virulence factors / Appl Environ Microbiol 67 : 3258 ~ 3263
Frans I / 2011 / Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention / J Fish Dis 34 : 643 ~ 661
Gonzalez SF / 2004 / Simultaneous detection of marine fish pathogens by using multiplex PCR and a DNA microarray / J Clin Microbiol 42 : 1414 ~ 1419
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