지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 107 - 115 (9page)
이용수
초록· 키워드
오류제보하기
In developing a useful tool to detect parasitic dynamics in an estuarine ecosystem, a denaturing high-performance liquid chromatography (DHPLC) assay was optimized by cloning plasmid DNA from the grass shrimp Palaemonetes pugio, and its two parasites,the trematode Microphallus turgidus and bopyrid isopod Probopyrus pandalicola. The optimal separation condition was an oven temperature of 57.9°C and 62-68% of buffer B gradient at a flow rate of 0.45 mL/min. A peptide nucleic acid blocking probe was designed to clamp the amplification of the host gene, which increased the amplification efficiency of genes with low copy numbers. Using the DHPLC assay with wild-type genomic, the assay could detect GC Gram positive bacteria and the bopyrid isopod (P. pandalicola). Therefore, the DHPLC assay is an effective tool for surveying parasitic dynamics in an estuarine ecosystem.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Ali MB / 1999 / Physicochemical characteristics and pollution level of Lake Nainital (U.P., India): role of macrophytes and phytoplankton in biomonitoring and phytoremediatio
Anderson GA / 1985 / Species Profiles: Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (Gulf of Mexico): Grass Shrimp : 13 ~
Baker GC / 2003 / Review and re-analysis of domain-specific 16S primers / J Microbiol Methods 55 : 541 ~ 555
Barlaan EA / 2005 / Profiling and monitoring of microbial populations by denaturing high-performance liquid chromatography / J Microbiol Meth 61 : 399 ~ 412
Blankenship LE / 2005 / Universal primers and PCR of gut contents to study marine invertebrate diets / Mol Ecol 14 : 891 ~ 899
Anderson GA / 1985 / Species Profiles: Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (Gulf of Mexico): Grass Shrimp : 13 ~
Baker GC / 2003 / Review and re-analysis of domain-specific 16S primers / J Microbiol Methods 55 : 541 ~ 555
Barlaan EA / 2005 / Profiling and monitoring of microbial populations by denaturing high-performance liquid chromatography / J Microbiol Meth 61 : 399 ~ 412
Blankenship LE / 2005 / Universal primers and PCR of gut contents to study marine invertebrate diets / Mol Ecol 14 : 891 ~ 899
이 논문의 저자 정보
최근 본 자료
댓글(0)
0