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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 22 - 30 (9page)
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초록· 키워드
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Vitellogenin (Vg) is the precursor of vitellin (Vn), which is the major yolk protein in nearly all oviparous species, including fish, amphibians, reptiles, and most invertebrates. It is one of the most important factors during reproduction, and numerous studies have shown that Vg genes are markers of the reproductive cycle and effecter genes induced by endocrine-disrupting chemicals (EDCs). Previously, we isolated two distinct cDNAs encoding vitellogenin homologs Pj-Vg1 and Pj-Vg2 from Pandalus shrimp Pandalopsis japonica. In this study, full-length genomic sequences of Pj-Vg1 and Pj-Vg2 were determined using a PCR-based genome walking strategy. Isolated Pj-Vg1 and Pj-Vg2 genes were 11,910 and 11,850 bp long, respectively. Both Pj-Vg genes had 15exons and 14 introns, and the splicing sites were also the same, suggesting that they arose via gene duplication. The similar structural characteristics of decapod Vg genes suggest that they are all orthologs that evolved from the same ancestral gene. Analysis of Pj-Vg1 and Pj-Vg2expression revealed that the relative copy numbers of Pj-Vg1 and Pj-Vg2 were similar in the hepatopancreas, whereas Pj-Vg2 transcripts were also detected in the ovary. Expression of both Pj-Vg genes was induced in hepatopancreas of mature individuals, whereas only Pj-Vg2transcripts were upregulated in the ovaries from mature animals, suggesting that both Pj-Vgs are important for oocyte development. A strong positive correlation was found between Pj-Vg1 and Pj-Vg2 transcripts in the same individual, indicating they are under the same control mechanisms.
Additionally, a positive correlation was found between ovarian and hepatopancreatic Pj-Vg2transcripts, suggesting that its dual expression is regulated by similar physiological conditions.
Knowledge of the similarities and differences between the two vitellogenin-like genes, Pj-Vg1and Pj-Vg2, would help us to understand their roles in reproduction and other physiological effects.
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참고문헌 (18)
참고문헌 신청
Abdu U / 2002 / The vitellogenin cDNA of Cherax quadricarinatus encodes a lipoprotein with calcium binding ability,and its expression is induced following the removal of the
Avarre JC / 2003 / Relationship between vitellogenin and vitellin in a marine shrimp(Penaeus semisulcatus)and molecular characterization of vitellogenin complementary DNAs /
Chen JS / 1997 / Extensive sequence conservation among insect,nematode,and vertebrate vitellogenins reveals ancient common ancestry / J Mol Evol 44 : 440 ~ 451
Gerber-Huber S / 1987 / Precursor-product relationship between vitellogenin and the yolk proteins as derived from the complete sequence of a Xenopus vitellogenin gene / Nucle
Jeon JM / 2010 / Characterization of two vitellogenin cDNAs from a Pandalus shrimp (Pandalopsis japonica): Expression in hepatopancreas is down-regulated by endosulfan exposu
Avarre JC / 2003 / Relationship between vitellogenin and vitellin in a marine shrimp(Penaeus semisulcatus)and molecular characterization of vitellogenin complementary DNAs /
Chen JS / 1997 / Extensive sequence conservation among insect,nematode,and vertebrate vitellogenins reveals ancient common ancestry / J Mol Evol 44 : 440 ~ 451
Gerber-Huber S / 1987 / Precursor-product relationship between vitellogenin and the yolk proteins as derived from the complete sequence of a Xenopus vitellogenin gene / Nucle
Jeon JM / 2010 / Characterization of two vitellogenin cDNAs from a Pandalus shrimp (Pandalopsis japonica): Expression in hepatopancreas is down-regulated by endosulfan exposu
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