지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 347 - 354 (8page)
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초록· 키워드
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Aeromonas salmonicida is an important fish pathogen commonly associated with furunculosis in salmonids. Typical A. salmonicida strains have the surface virulence A-layer protein, a major virulence determinant encoded by the vapA gene. In this study, 880 chum salmon Oncorhynchus keta were collected from the east coast of Korea during 2006-2011, including 560 wild adults and 320 artificially hatched fry pools, and the presence of typical A. salmonicida was examined by PCR using the typical A. salmonicida-specific vapA gene primers. The results demonstrated that 34.5% of the samples (304/880 samples) were PCR positive, implying that a typical A. salmonicida infection is highly prevalent among chum salmon in Korea. Twenty typical A. salmonicida isolates were recovered based on their brown pigmentation on Trypticase Soy Agar (TSA) plates, which indicates the existence of the A-layer protein. Further biochemical analyses with the four randomly selected typical A. salmonicida isolates revealed some variations in their amino acid decarboxylation and carbohydrate fermentation activity. A phylogenetic analysis based on the entire vapA gene sequence suggested that the A. salmonicida isolates from chum salmon were clustered with those isolated from Atlantic salmon in Europe. Further study is needed to resolve such an interesting relationship in detail.
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참고문헌 (22)
참고문헌 신청
Abbott SL / 2003 / The genus Aeromonas:biochemical characteristics,atypical reactions,and phenotypic identification schemes / J Clin Microbiol 41 : 2348 ~ 2357
Austin B / 2007 / Bacterial Fish Pathogens:Diseases of Farmed and Wild Fish.4th ed / Springer-Praxis : 25 ~ 89
Byers HK / 2002 / PCR-based assays for the fish pathogen Aeromonas salmonicida.I.Evaluation of three PCR primer sets for detection and identification / Dis Aquat Org 49 : 129
Cipriano RC / 1988 / Selection for virulence in the fish pathogen Aeromonas salmonicida,using Coomassie Brilliant Blue agar / J Wildl Dis 24 : 672 ~ 678
Fryer JL / 1988 / Isolation of Aeromonas salmonicida from masu salmon in the Republic of Korea / J Wildl Dis 24 : 364 ~ 365
Austin B / 2007 / Bacterial Fish Pathogens:Diseases of Farmed and Wild Fish.4th ed / Springer-Praxis : 25 ~ 89
Byers HK / 2002 / PCR-based assays for the fish pathogen Aeromonas salmonicida.I.Evaluation of three PCR primer sets for detection and identification / Dis Aquat Org 49 : 129
Cipriano RC / 1988 / Selection for virulence in the fish pathogen Aeromonas salmonicida,using Coomassie Brilliant Blue agar / J Wildl Dis 24 : 672 ~ 678
Fryer JL / 1988 / Isolation of Aeromonas salmonicida from masu salmon in the Republic of Korea / J Wildl Dis 24 : 364 ~ 365
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