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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 265 - 275 (11page)
이용수
초록· 키워드
오류제보하기
G Protein-coupled receptors (GPCRs) mediating wide ranges of physiological responses is one of the most attractive targets for drug development. Rhodopsin, a dim-light photoreceptor, has been extensively used as a model system for structural and functional study of GPCRs. Fish have rhodopsin finely-tuned to their habitats where the intensity and the wavelength of lights are changed depending on its water-depth. To study the detailed molecular characteristics of GPCR architecture and to understand the fishery light-sensing system, genes encoding rod opsins were isolated from fishes living under different photic environments. Full-length rod opsin genes were obtained by combination of PCR amplification and DNA walking strategy of genomic DNA isolated from olive flounder, P. olivaceus, Japanese eel, A. japonica, and Common carp C. carpio. Deduced amino acid sequences showed a typical feature of rod opsins including the sites for Schiff's base formation (Lys296) and its counter ion (Glu113), disulfide formation (Cys110 and Cys187), and palmitoylation (Cys322 and Cys323) although Cys322 is replaced by Phe in Japanese eel. Comparison of opsins by amino acid sequence alignment indicated the closest similarity between P. olivaceus and H. hippoglossus (94%), A. japonica and A. anguilla (98%), and C. carpio and C. auratus (95%), respectively.
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등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Archer S / 1995 / The molecular basis for the green-blue sensitivity shift in the rod visual pigments of the European eel / Proc. Roy. Soc. Lond 262 : 289 ~ 295
Bowmaker JK / 1999 / Molecular biology of photoreceptor spectral sensitivity. In adaptive mech-anisms in the ecology of vision / Dordrecht: Kluwer Academic Publisher : 439 ~ 462
Cherezov V / 2007 / High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor / Science 318 : 1258 ~ 1266
Davies WL / 2007 / Functional characterization, tuning, and regulation of visual pig- ment gene expression in an anadromous lamprey / FASEB J. 21 : 2713 ~ 2724
Fitzgibbon J / 1995 / The rhodopsin-encoding gene of bony fish lacks introns / Gene 164 : 273 ~ 277
Bowmaker JK / 1999 / Molecular biology of photoreceptor spectral sensitivity. In adaptive mech-anisms in the ecology of vision / Dordrecht: Kluwer Academic Publisher : 439 ~ 462
Cherezov V / 2007 / High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor / Science 318 : 1258 ~ 1266
Davies WL / 2007 / Functional characterization, tuning, and regulation of visual pig- ment gene expression in an anadromous lamprey / FASEB J. 21 : 2713 ~ 2724
Fitzgibbon J / 1995 / The rhodopsin-encoding gene of bony fish lacks introns / Gene 164 : 273 ~ 277
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