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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 455 - 471 (17page)
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Objectives: This experiment was done for investigating antimicrobial activity of Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, Gardenia Jasminoides extract against Staphylococcus aureus.
Methods: After administering S. aureus on a bacterial culture media plate, antimicrobial activity was tested by dripping 80 μl diluted Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, and Gardenia Jasminoides extract (100%, 50%, 10%, 1%) on plates that were cultivated for a span of time from 16 to 72 hours. Also, minimal inhibitory concentration (MIC) was tested by dripping the minimum dilution density solution that has antimicrobial activity between 80 μl and 20 μl (80 μl, 60 μl, 40 μl, 20 μl) in measure of density. Antimicrobial activity of Hwangryunheadok-tang and four kinds of extracts against S. aureus was continually displayed.
Results:1. S. aureus (Standard Microorganism, ATCC)(1) Antimicrobial activity was displayed for Hwangryunheadok-tang, Scutellariae Radix extract, and Phellodendri Cortex extract respectively in the undiluted solution and 50% of diluted magnification. Gardenia Jasminoides extract showed its activity only in the undiluted solution; Coptis Rhizoma extract showed its activity down to 10% of diluted magnification. The antimicrobial activity of the undiluted solution was increased when the volume of inoculation increased. But, there was no difference when time was extended for cultivation.
(2) MIC of Hwangryunheadok-tang, Scutellariae Radix extract was 50%, 20 μl. Coptis Rhizoma extract was 10%, 20 μl, Phellodendri Cortex extract was 50%, 80 μl and Gardenia Jasminoides extract was 100%, 60 μl.
2. S. aureus isolated from diarrheal patients(1) When compared to standard microorganism, MIC has decreased. As a result, their antimicrobial activity has increased.
(2) Antimicrobial activity of Hwangryunheadok-tang and four extracts was continually shown in extending of the time, 16, 24 and 72 hours.
Conclusions: The author comes to the conclusion that Hwangryunheadok-tang, and four kinds of extracts have antimicrobial activity against S. aureus. Especially, when comparing standard microorganisms with S. aureus isolated from diarrheal patients, some cases showed that antimicrobial activity of all the extracts were better than antibiotics. Thus, if a further study is performed, the use of Hwangryunheadok-tang, and four kinds of extracts will be valuable and beneficial in clinical treatments.
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참고문헌 (33)
참고문헌 신청
Alterkruse SF / 1997 / Emerrging foodborne disease / Emerg Infedt Dis 3 : 285 ~ 293
Hiramatsu K / 1997 / Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin / Lancet 350 (9092) : 1670 ~ 1673
Hong ND / 1982 / Studies on the efficacy of combined preparation of crude drugs / Kor J Pharmacogn 13 (1) : 26 ~ 32
Oldfield EC / 2001 / Emerging foodborne pathogens: keeping your patients and your families safe / Rev Gastroenterol Disorders 1 (4) : 177 ~ 186
강성구 / 2006 / 인체생물학 / 아카데미서적 : 363 ~ 367
Hiramatsu K / 1997 / Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin / Lancet 350 (9092) : 1670 ~ 1673
Hong ND / 1982 / Studies on the efficacy of combined preparation of crude drugs / Kor J Pharmacogn 13 (1) : 26 ~ 32
Oldfield EC / 2001 / Emerging foodborne pathogens: keeping your patients and your families safe / Rev Gastroenterol Disorders 1 (4) : 177 ~ 186
강성구 / 2006 / 인체생물학 / 아카데미서적 : 363 ~ 367
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