지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Background : Despite recent advances in cancer management, prognosis of ovarian cancer is poor. Anticancer effects of herbal medicine, such as Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger, have been reported in treatment of ovarian and cervical cancers, but the systematic approaches to explain their molecular mechanism(s) have not yet been established. Objectives : To establish a basis of understanding for anti-cancer mechanisms of herbal medicine, we profiled protein expression in human ovarian and cervical cancer cells treated with the extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh and Orostachys japonicus A. Berger. Methods : Human ovarian cancer cell line NIH:OVCAR-3, and human cervical cancer cell line HeLa were employed in the present study. Whole protein was obtained from the cells harvested at 48 hours after the treatment with herbal water-extract, and analyzed by 2DE-based proteomic approach. Results : Various changes of protein expression induced by the herbal treatment were monitored : down-regulation of molecular chaperone (calreticulin variant), glycolytic enzymes (D-3-phosphoglycerate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase and alpha-enolase), RNA processing molecules (hnRNP A2/B1), and antioxidant protein (peroxiredoxin 1). Conclusions : Repression of glycolysis has been accepted as the mechanism to increase anticancer reagent's effect. Thus, down-regulation of glycolytic enzymes by the herbal extracts suggested a possible synergistic effect of herbs in the presence of platinum-based therapeutics. In further study, as well as the synergistic effect of the herbs, it has to be further validated whether artificial regulation of hnRNP A2/B1 in ovarian cancer cells affects various cancer survival factors, since RNA processing can be interrupted by deranged expression of hnRNP subtypes, and it results in an inhibition of cancer cell growth.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Joshi G / 2010 / Alterations in brain antioxidant enzymes and redox proteomic identification of oxidized brain proteins induced by the anti-cancer drug adriamycin: implications for oxidative stress- mediated chemobrain / Neuroscience 166(3):796~807
Can CY / 2010 / Apoptotic B16-F1 cells coated with recombinant calreticulin mediated anti-tumor immune response in mice / Chinese Journal of Cancer Research 22 (4) : 253 ~ 2
Chen CN / 2009 / Identification of calreticulin as a prognosis marker and angiogenic regulator in human gastric cancer / Ann Surg Oncol 16 (2) : 524 ~ 533
Chhipa RR / 2009 / Prx1 Enhances Androgen Receptor Function in Prostate Cancer Cells by Increasing Receptor Affinity to Dihydrotestost
Cho WC / 2007 / Application of proteomics in Chinese medicine research / Am J Chin Med 35 (6) : 911 ~ 922
Can CY / 2010 / Apoptotic B16-F1 cells coated with recombinant calreticulin mediated anti-tumor immune response in mice / Chinese Journal of Cancer Research 22 (4) : 253 ~ 2
Chen CN / 2009 / Identification of calreticulin as a prognosis marker and angiogenic regulator in human gastric cancer / Ann Surg Oncol 16 (2) : 524 ~ 533
Chhipa RR / 2009 / Prx1 Enhances Androgen Receptor Function in Prostate Cancer Cells by Increasing Receptor Affinity to Dihydrotestost
Cho WC / 2007 / Application of proteomics in Chinese medicine research / Am J Chin Med 35 (6) : 911 ~ 922
이 논문의 저자 정보
최근 본 자료
댓글(0)
0