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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Background and Objective : The prospects for developing an anti-apoptotic natural component or a compound that exerts a neuroprotective effect with few or no side effects for the treatment of neurodegenerative disease appear favorable. In the present study, we evaluated the effects of the methanol extract of Phellodendri Cortex (PC extract) on 1-methyl-4-phenyl pyridinium(MPP+)-induced apoptosis in PC-12 cells.
Materials and Metohds : We used the methanol extract of Phellodendri Cortex (PC extract). PC-12 cells was cultured by RPMZ-1640. We found the PC extract's gene expression(bax, bcl-s) by using RT-PCR. And we examined the PC extract's protein expression(bcl-2, bax, cytochrome c, poly(ADP-ribose) polymerase(PARP), caspase-3) by SDS-PAGE and Western blot.
Results : Apoptosis in MPP+-induced PC-12 cells was accompanied by an increased bax/bcl-2 ratio, release of cytochrome c to the cytosol and the activation of caspase-3. PC extract inhibited the down-regulation of bcl-2 and the up-regulation of bax, as well as the release of mitochondrial cytochrome c into the cytosol. In addition, PC extract attenuated caspase-3 activation and cleavage of poly (ADP-ribose) polymerase (PARP).
Conclusion : These results suggest that the neuroprotective potentials of PC extract against MPP+-induced apoptosis can be, at least partially, ascribed to its anti-apoptotic effects in PC-12cells.
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참고문헌 (29)
참고문헌 신청
?廷賢 / 1998 / 萬病回春 / 北京: 人民衛生出版社 : 14 ~
Camins A / 2008 / Apoptotic mechanisms involved in neurodegenerative diseases: experimental and therapeutic approaches / Methods Find Exp Clin Pharmacol 30 (1) : 43 ~ 65
Cao F / 2003 / Experimental study on inhibition of neuronal toxical effect of levodopa by ginkgo biloba extract on Parkinson disease in rats / J Huazhong Univ Sci Technolog
H Mori / 1995 / Principle of the bark of Phellodendron amurense to suppress the cellular immune response: effect of phellodendrine on cellular and humoral immune responses /
Jenner P / 1998 / Understanding cell death in Parkinson's disease / Ann Neurol 3 : S72 ~ S84
Camins A / 2008 / Apoptotic mechanisms involved in neurodegenerative diseases: experimental and therapeutic approaches / Methods Find Exp Clin Pharmacol 30 (1) : 43 ~ 65
Cao F / 2003 / Experimental study on inhibition of neuronal toxical effect of levodopa by ginkgo biloba extract on Parkinson disease in rats / J Huazhong Univ Sci Technolog
H Mori / 1995 / Principle of the bark of Phellodendron amurense to suppress the cellular immune response: effect of phellodendrine on cellular and humoral immune responses /
Jenner P / 1998 / Understanding cell death in Parkinson's disease / Ann Neurol 3 : S72 ~ S84
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