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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제45권 제2호
- 발행연도
- 2015.1
- 수록면
- 38 - 45 (8page)
이용수
초록· 키워드
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Purpose: The purpose of this study was to evaluate the effect of photodynamic therapy (PDT) using erythrosine and a green light emitting diode (LED) light source on biofilms of Aggregatibacter actinomycetemcomitans attached to resorbable blasted media (RBM) and sandblasted, large-grit, acid-etched (SLA) titanium surfaces in vitro.
Methods: RBM and SLA disks were subdivided into four groups, including one control group and three test groups (referred to as E0, E30, E60), in order to evaluate the effect of PDT on each surface. The E0 group was put into 500 μL of 20 μM erythrosine for 60 seconds without irradiation, the E30 group was put into erythrosine for 60 seconds and was then irradiated with a LED for 30 seconds, and the E60 group was put into erythrosine for 60 seconds and then irradiated with a LED for 60 seconds. After PDT, sonication was per\-formed in order to detach the bacteria, the plates were incubated under anaerobic condi\-tions on brucella blood agar plates for 72 hours at 37°C, and the number of colony-forming units (CFUs) was determined.
Results: Significant differences were found between the control group and the E30 and E60 groups (P<0.05). A significantly lower quantity of CFU/mL was found in the E30 and E60 groups on both titanium disk surfaces. In confocal scanning laser microscopy images, increased bacterial death was observed when disks were irradiated for a longer period of time.
Conclusions: These findings suggest that PDT using erythrosine and a green LED is effective in reducing the viability of A. actinomycetemcomitans attached to surface-modified titanium in vitro.
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참고문헌 (24)
참고문헌 신청
Alcoforado GA / 1991 / Microbial aspects of failing osseointegrated dental implants in humans / J Parodontol 10 : 11 ~ 18
Becker W / 1990 / Clinical and microbiologic findings that may contribute to dental implant failure / Int J Oral Maxillofac Implants 5 : 31 ~ 38
Burgers R / 2010 / In vivo and in vitro biofilm formation on two different titanium implant surfaces / Clin Oral Implants Res 21 : 156 ~ 164
Dortbudak O / 2001 / Lethal photosensitization for decontamination of implant surfaces in the treatment of peri-implantitis / Clin Oral Implants Res 12 : 104 ~ 108
Haas R / 1997 / Elimination of bacteria on different implant surfaces through photosensitization and soft laser. An in vitro study / Clin Oral Implants Res 8 : 249 ~ 254
Becker W / 1990 / Clinical and microbiologic findings that may contribute to dental implant failure / Int J Oral Maxillofac Implants 5 : 31 ~ 38
Burgers R / 2010 / In vivo and in vitro biofilm formation on two different titanium implant surfaces / Clin Oral Implants Res 21 : 156 ~ 164
Dortbudak O / 2001 / Lethal photosensitization for decontamination of implant surfaces in the treatment of peri-implantitis / Clin Oral Implants Res 12 : 104 ~ 108
Haas R / 1997 / Elimination of bacteria on different implant surfaces through photosensitization and soft laser. An in vitro study / Clin Oral Implants Res 8 : 249 ~ 254
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