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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제34권 제2호
- 발행연도
- 2004.1
- 수록면
- 317 - 332 (16page)
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초록· 키워드
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Osteoblasts from alveolar bone may have an important role in the bone regeneration for periodontium, but their culture and characterization are not determined yet. The purpose of this study was to investigate the biological characteristics of primary explant cultured osteoblasts(PECO) from alveolar bone. Osteoblasts were isolated and cultured from alveolar socket of extracted tooth in children. To compare the characteristics, osteoblasts and gingival fibroblasts were cultured with DMEM at 37℃, 5% CO2, 100% humidity incubator, and human fetal osteoblasts cell line(hFOB1) were cultured with DMEM at 34℃, 5%, CO2, 100% humidity incubator. To characterize the isolated bone cells, morphologic change, cell proliferation and differentiation were measured. Morphology of PECO was small round body or cuboidal shape on inverted microscope and was similar with hFOB1. PECO became polygonal shape with stellate and had an amorphous shape at 9th passage in culture. PECO had significantly higher activity than that of gingival fibroblasts and hFOB1 in alkaline phosphatase activity. The expression of osteocalcin and bone sialoprotein in PECO was notably increased when compared with hFOB1 and gingival fibroblasts. These result indicated that PECO from alveolar bone in children has an obvious characteristics of osteoblast, may be applied for the regeneration of bone.
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참고문헌 (26)
참고문헌 신청
/ 1972. / Bone resorption induced by epithelial and connective tissue from human gingiva in vitro. J Dent Res 51 : 1683 ~ 1689
/ 1972. / Human allografts of iliac cancellous bone and marrow in periodontal osseous defects. Ⅱ. Clinical observations. J Periodontol 43 : 67 ~ 81
/ 1972. / In vitro culture of periodontal ligament cells. J Dent Res 51 : 953 ~ 959
/ 1978 / Binding of chemotatic collagen-derived peptides to fibroblasts The relationship to fibroblast chemotaxis : 916 ~ 926
/ 1980. / Connective tissue regeneration to periodontally diseased teeth. A histological study. J Periodont Res 15 : 1 ~ 9
/ 1972. / Human allografts of iliac cancellous bone and marrow in periodontal osseous defects. Ⅱ. Clinical observations. J Periodontol 43 : 67 ~ 81
/ 1972. / In vitro culture of periodontal ligament cells. J Dent Res 51 : 953 ~ 959
/ 1978 / Binding of chemotatic collagen-derived peptides to fibroblasts The relationship to fibroblast chemotaxis : 916 ~ 926
/ 1980. / Connective tissue regeneration to periodontally diseased teeth. A histological study. J Periodont Res 15 : 1 ~ 9
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