지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제41권 제4호
- 발행연도
- 2011.1
- 수록면
- 167 - 175 (9page)
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초록· 키워드
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Purpose: Periodontal ligament (PDL) cell differentiation into osteoblasts is important in bone formation. Bone formation is a complex biological process and involves several tightly regulated gene expression patterns of bone-related proteins. The expression patterns of bone related proteins are regulated in a temporal manner both in vivo and in vitro. The aim of this study was to observe the gene expression profile in PDL cell proliferation, differentiation, and mineralization in vitro.
Methods: PDL cells were grown until confluence, which were then designated as day 0, and nodule formation was induced by the addition of 50 μg/mL ascorbic acid, 10 mM β-glycerophosphate, and 100 nM dexamethasone to the medium. The dishes were stained with Alizarin Red S on days 1, 7, 14, and 21. Real-time polymerase chain reaction was performed for the detection of various genes on days 0, 1, 7, 14, and 21.
Results: On day 0 with a confluent monolayer, in the active proliferative stage, c-myc gene expression was observed at its maximal level. On day 7 with a multilayer, alkaline phosphatase, bone morphogenetic protein (BMP)-2, and BMP-4 gene expression had increased and this was followed by maximal expression of osteocalcin on day 14 with the initiation of nodule mineralization. In relationship to apoptosis, c-fos gene expression peaked on day 21 and was characterized by the post-mineralization stage. Here, various genes were regulated in a temporal manner during PDL fibroblast proliferation, extracellular matrix maturation, and mineralization. The gene expression pattern was similar.
Conclusions: We can speculate that the gene expression pattern occurs during PDL cell proliferation, differentiation, and mineralization. On the basis of these results, it might be possible to understand the various factors that influence PDL cell proliferation, extracellular matrix maturation, and mineralization with regard to gene expression patterns.
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참고문헌 (24)
참고문헌 신청
Aubin JE / 1993 / Osteoblastic cell lineage In Cellular and molecular biology of bone / Academic Press : 1 ~ 44
Cho MI / 1992 / In vitro formation of mineralized nodules by periodontal ligament cells from the rat / Calcif Tissue Int 50 : 459 ~ 467
Choi JY / 1996 / Expression patterns of bone-related proteins during osteoblastic differentiation in MC3T3-E1 cells / J Cell Biochem 61 : 609 ~ 618
Hughes FJ / 1995 / The effects of bone morphogenetic protein-2, -4, and -6 on differentiation of rat osteoblast cells in vitro / Endocrinology 136 : 2671 ~ 2677
Ivanovski S / 2001 / Expression of bone associated macromolecules by gingival and periodontal ligament fibroblasts / J Periodontal Res 36 : 131 ~ 141
Cho MI / 1992 / In vitro formation of mineralized nodules by periodontal ligament cells from the rat / Calcif Tissue Int 50 : 459 ~ 467
Choi JY / 1996 / Expression patterns of bone-related proteins during osteoblastic differentiation in MC3T3-E1 cells / J Cell Biochem 61 : 609 ~ 618
Hughes FJ / 1995 / The effects of bone morphogenetic protein-2, -4, and -6 on differentiation of rat osteoblast cells in vitro / Endocrinology 136 : 2671 ~ 2677
Ivanovski S / 2001 / Expression of bone associated macromolecules by gingival and periodontal ligament fibroblasts / J Periodontal Res 36 : 131 ~ 141
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