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학술저널
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대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제41권 제5호
발행연도
2011.1
수록면
218 - 226 (9page)

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Purpose: Micro-computed tomography (micro-CT) has been widely used in the evaluation of regenerated bone tissue but the reliability of micro-CT has not yet been established. This study evaluated the correlation between histomorphometric analysis and micro-CT analysis in performing new bone formation measurement. Methods: Critical-size calvarial defects were created using a 8 mm trephine bur in a total of 24 Sprague-Dawley rats, and collagen gel mixed with autogenous rat bone marrow stromal cells (BMSCs) or autogenous rat BMSCs transduced by adenovirus containing bone morphogenic protein-2 (BMP-2) genes was loaded into the defect site. In the control group, collagen gel alone was loaded into the defect. After 2 and 4 weeks, the animals were euthanized and calvaria containing defects were harvested. Micro-CT analysis and histomorphometric analysis of each sample were accomplished and the statistical evaluation about the correlation between both analyses was performed. Results: New bone formation of the BMP-2 group was greater than that of the other groups at 2 and 4 weeks in both histomorphometric analysis and micro-CT analysis (P=0.026, P=0.034). Histomorphometric analysis of representative sections showed similar results to histomorphometric analysis with a mean value of 3 sections. Measurement of new bone formation was highly correlated between histomorphometric analysis and micro-CT analysis, especially at the low lower threshold level at 2 weeks (adjusted r2=0.907, P<0.001). New bone formation of the BMP-2 group analyzed by micro-CT tended to decline sharply with an increasing lower threshold level, and it was statistically significant (P<0.001). Conclusions: Both histomorphometric analysis and micro-CT analysis were valid methods for measurement of the new bone in rat calvarial defects and the ability to detect the new bone in micro-CT analysis was highly influenced by the threshold level in the BMP-2 group at early stage.
#Bone morphogenic protein-2 #Gene therapy #Histology #X-Ray microtomography.

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참고문헌 (21)

참고문헌 신청
Chang PC / 2010 / Franco LM, et al. PDGF-B gene therapy accelerates bone engineering and oral implant osseointegration / Gene Ther 17 : 95 ~ 104 google schola Chang SC / 2004 / Cranial repair using BMP-2 gene engineered bone marrow stromal cells / J Surg Res 119 : 85 ~ 91 google schola Evans C / 2011 / Gene therapy for the regeneration of bone / Injury 42 : 599 ~ 604 google schola Fischer J / 2011 / Future of local bone regeneration - Protein versus gene therapy / J Craniomaxillofac Surg 39 : 54 ~ 64 google schola Gielkens PF / 2008 / A comparison of micro-CT, microradiography and histomorphometry in bone research / Arch Oral Biol 53 : 558 ~ 566 google schola

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