지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제42권 제3호
- 발행연도
- 2012.1
- 수록면
- 95 - 104 (10page)
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초록· 키워드
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Purpose: The purpose of this study was to determine whether increasing the Ti6Al4V surface oxide negative charge through heat (600°C) or radiofrequency plasma glow discharge (RFGD) pretreatment, with or without a subsequent coating with fibronectin,stimulated osteoblast gene marker expression in the MC3T3 osteoprogenitor cell line.
Methods: Quantitative real-time polymerase chain reaction was used to measure changes over time in the mRNA levels for osteoblast gene markers, including alkaline phosphatase, bone sialoprotein, collagen type I (α1), osteocalcin, osteopontin and parathyroid hormone-related peptide (PTH-rP), and the osteoblast precursor genes Runx2 and osterix.
Results: Osteoprogenitors began to differentiate earlier on disks that were pretreated with heat or RFGD. The pretreatments increased gene marker expression in the absence of a fibronectin coating. However, pretreatments increased osteoblast gene expression for fibronectin-coated disks more than uncoated disks, suggesting a surface oxide-mediated specific enhancement of fibronectin’s bioactivity. Heat pretreatment had greater effects on the mRNA expression of genes for PTH-rP, alkaline phosphatase and osteocalcin while RFGD pretreatment had greater effects on osteopontin and bone sialoprotein gene expression.
Conclusions: The results suggest that heat and RFGD pretreatments of the Ti6Al4V surface oxide stimulated osteoblast differentiation through an enhancement of (a) coated fibronectin’s bioactivity and (b) the bioactivities of other serum or matrix proteins. The quantitative differences in the effects of the two pretreatments on osteoblast gene marker expression may have arisen from the unique physico-chemical characteristics of each resultant oxide surface. Therefore, engineering the Ti6Al4V surface oxide to become more negatively charged can be used to accelerate osteoblast differentiation through fibronectin-dependent and independent mechanisms.
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참고문헌 (23)
참고문헌 신청
Adell R / 1981 / A 15-year study of osseointegrated implants in the treatment of the edentulous jaw / Int J Oral Surg 10 : 387 ~ 416
Adell R / 1990 / Long-term follow-up study of osseointegrated implants in the treatment of totally edentulous jaws / Int J Oral Maxillofac Implants 5 : 347 ~ 359
Bentley KL / 1985 / Fibronectin binding properties of bacteriologic petri plates and tissue culture dishes / J Biomed Mater Res 19 : 757 ~ 769
Grossmann Y / 2007 / Success and survival of single dental implants placed in sites of previously failed implants / J Periodontol 78 : 1670 ~ 1674
Hardt CR / 2002 / Outcome of implant therapy in relation to experienced loss of periodontal bone support: a retrospective 5-year study / Clin Oral Implants Res 13 : 488 ~ 494
Adell R / 1990 / Long-term follow-up study of osseointegrated implants in the treatment of totally edentulous jaws / Int J Oral Maxillofac Implants 5 : 347 ~ 359
Bentley KL / 1985 / Fibronectin binding properties of bacteriologic petri plates and tissue culture dishes / J Biomed Mater Res 19 : 757 ~ 769
Grossmann Y / 2007 / Success and survival of single dental implants placed in sites of previously failed implants / J Periodontol 78 : 1670 ~ 1674
Hardt CR / 2002 / Outcome of implant therapy in relation to experienced loss of periodontal bone support: a retrospective 5-year study / Clin Oral Implants Res 13 : 488 ~ 494
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