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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치과보철학회 The Journal of Advanced of Prosthodontics The Journal of Advanced of Prosthodontics 제6권 제5호
- 발행연도
- 2014.1
- 수록면
- 351 - 360 (10page)
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초록· 키워드
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PURPOSE. The aim of present study was to identify characteristic and response of mouse bone marrow (BM) derived low-adherent bone marrow mesenchymal stem cells (BMMSCs) obtained by quantification of extracellular matrix (ECM). MATERIALS AND METHODS. Non-adherent cells acquired by ECM coated dishes were termed low-adherent BMMSCs and these cells were analyzed by in vitro and in vivo methods, including colony forming unit fibroblast (CFU-f), bromodeoxyuridine (BrdU), multi-potential differentiation, flow cytometry and transplantation into nude mouse to measure the bone formation ability of these low-adherent BMMSCs. Titanium (Ti) discs with machined and anodized surfaces were prepared. Adherent and low-adherent BMMSCs were cultured on the Ti discs for testing their proliferation. RESULTS. The amount of CFU-f cells was significantly higher when non-adherent cells were cultured on ECM coated dishes, which was made by 7 days culturing of adherent BMMSCs. Low-adherent BMMSCs had proliferation and differentiation potential as adherent BMMSCs in vitro. The mean amount bone formation of adherent and low-adherent BMMSCs was also investigated in vivo. There was higher cell proliferation appearance in adherent and low-adherent BMMSCs seeded on anodized Ti discs than machined Ti discs by time. CONCLUSION. Low-adherent BMMSCs acquired by ECM from nonadherent cell populations maintained potential characteristic similar to those of the adherent BMMSCs and therefore could be used effectively as adherent BMMSCs in clinic.
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참고문헌 (23)
참고문헌 신청
[학술지(정기간행물)]
Akiyama K. / 2012 / Characterization of bone marrow derived mesenchymal stem cells in suspension / Stem Cell Res Ther 3 : 40 ~
Blazsek I. / 2000 / Ontogenic emergence of the hematon, a morphogenetic stromal unit that supports multipotential hematopoietic progenitors in mouse bone marrow / Blood 96 :
Chen XD. / 2007 / Extracellular matrix made by bone marrow cells facilitates expansion of marrow-derived mesenchymal progenitor cells and prevents their differentiation into
Clarke E. / 1991 / Stromal colonies can be grown from the non-adherent cells in human long-term bone marrow cultures / Eur J Haematol 46 : 296 ~ 300
Akiyama K. / 2012 / Characterization of bone marrow derived mesenchymal stem cells in suspension / Stem Cell Res Ther 3 : 40 ~
Blazsek I. / 2000 / Ontogenic emergence of the hematon, a morphogenetic stromal unit that supports multipotential hematopoietic progenitors in mouse bone marrow / Blood 96 :
Chen XD. / 2007 / Extracellular matrix made by bone marrow cells facilitates expansion of marrow-derived mesenchymal progenitor cells and prevents their differentiation into
Clarke E. / 1991 / Stromal colonies can be grown from the non-adherent cells in human long-term bone marrow cultures / Eur J Haematol 46 : 296 ~ 300
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