Statement of problem: In its preceding work, change in surface characteristics were
investigated in consideration that both microtopograpy and macroscopic configuration of
implants surface are two of the most important factors, in that they can construct agreeable environment
by raising surface energy, to affect osseointegration and biocompatibility explained by
cell proliferation.
Purpose: This study focused on examining cytocompatibility of dental implants materials Ti-
Ag alloys, of which mechanical and electrochemical superiority to cp-Ti or Ti6Al4V were verified,
in comparison with that of cp-Ti, and Ti6Al4V.
Materials and methods: In this regard, MTT tests for L-929, the fibroblast connective tissues
and cell proliferation tests for osteoprogenitor cells, MC3T3-E1 were performed on cp-Ti, Ti6Al4V,
and Ti-Ag alloys following thermal oxidation according to appropriate heat treatment temperature(
untreated, 400, 600, 800℃) and heat treatment duration(untreated, 0.5, 1, 4 hr).
Results: The MTT tests on fibroblasts L-929 resulted in cell viability of over 90% in all experimental
group entities, where, especially, the 100% of the viability for Ti-Ag alloys specimens accounted
for the slightest adverse effect of ions release from those alloys on the cell. In MC3T3-E1 proliferation
tests, the population of cells in the experimental group was roughly increased as experimentation
proceeded, after two to four days. Proliferation showed highest viability for most of specimens,
including Ti2.0Ag, treated at 600℃.
Conclusion: In conclusion, it is the heat treatment temperature, not the duration that has considerable
effects on thermal oxidation of specimens. Ti-Ag alloys treated at 600℃ proved to have
the best surface morphology as well as cytocompatibility when compared with Ti or Ti6Al4V for
short-term biocompatibility tests.
Statement of problem: In its preceding work, change in surface characteristics were
investigated in consideration that both microtopograpy and macroscopic configuration of
implants surface are two of the most important factors, in that they can construct agreeable environment
by raising surface energy, to affect osseointegration and biocompatibility explained by
cell proliferation.
Purpose: This study focused on examining cytocompatibility of dental implants materials Ti-
Ag alloys, of which mechanical and electrochemical superiority to cp-Ti or Ti6Al4V were verified,
in comparison with that of cp-Ti, and Ti6Al4V.
Materials and methods: In this regard, MTT tests for L-929, the fibroblast connective tissues
and cell proliferation tests for osteoprogenitor cells, MC3T3-E1 were performed on cp-Ti, Ti6Al4V,
and Ti-Ag alloys following thermal oxidation according to appropriate heat treatment temperature(
untreated, 400, 600, 800℃) and heat treatment duration(untreated, 0.5, 1, 4 hr).
Results: The MTT tests on fibroblasts L-929 resulted in cell viability of over 90% in all experimental
group entities, where, especially, the 100% of the viability for Ti-Ag alloys specimens accounted
for the slightest adverse effect of ions release from those alloys on the cell. In MC3T3-E1 proliferation
tests, the population of cells in the experimental group was roughly increased as experimentation
proceeded, after two to four days. Proliferation showed highest viability for most of specimens,
including Ti2.0Ag, treated at 600℃.
Conclusion: In conclusion, it is the heat treatment temperature, not the duration that has considerable
effects on thermal oxidation of specimens. Ti-Ag alloys treated at 600℃ proved to have
the best surface morphology as well as cytocompatibility when compared with Ti or Ti6Al4V for
short-term biocompatibility tests.