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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 342 - 352 (11page)
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초록· 키워드
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Background: Eicosanoids are metabolites of arachidonic acid that are rapidly biosynthesized and degraded during inflammation, and their metabolic changes reveal altered enzyme expression following drug treatment. We developed an eicosanoid profiling method and evaluated their changes on drug treatment.
Methods: Simultaneous quantitative profiling of 32 eicosanoids in liver S9 fractions obtained from rabbits with carrageenan-induced inflammation was performed and validated by liquid chromatography-mass spectrometry coupled to anion-exchange solid-phase purification.
Results: The limit of quantification for the devised method ranged from 0.5 to 20.0 ng/mg protein, and calibration linearity was achieved (R2>0.99). The precision (% CV) and accuracy (% bias) ranged from 4.7 to 10.3% and 88.4 to 110.9%, respectively, and overall recoveries ranged from 58.0 to 105.3%. Our method was then applied and showed that epitestosterone treatment reduced the levels of all eicosanoids that were generated by cyclooxygenases and lipoxygenases.
Conclusions: Quantitative eicosanoid profiling combined with in vitro metabolic assays may be useful for evaluating metabolic changes affected by drugs during eicosanoid metabolism.
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등록된 정보가 없습니다.
참고문헌 (37)
참고문헌 신청
Musiek ES / 2005 / Cyclopentenone eicosanoids as mediators of neurodegeneration: a pathogenic mechanism of oxidative stress-mediated and cyclooxygenase-mediated neurotoxicity / Brain Pathol 15:149~158
Wymann MP / 2008 / Lipid signalling in disease / Nat Rev Mol Cell Biol 9:162~176
Serhan CN / 2008 / Resolving inflammation: dual anti-inflammatory and pro-resolution lipid mediators / Nat Rev Immunol 8:349~361
Balazy M / 2004 / Eicosanomics: targeted lipidomics of eicosanoids in biological systems / Prostaglandins Other Lipid Mediat 73:173~180
Theken KN / 2011 / Activation of the acute inflammatory response alters cytochrome P450 expression and eicosanoid metabolism / Drug Metab Dispos 39:22~29
Wymann MP / 2008 / Lipid signalling in disease / Nat Rev Mol Cell Biol 9:162~176
Serhan CN / 2008 / Resolving inflammation: dual anti-inflammatory and pro-resolution lipid mediators / Nat Rev Immunol 8:349~361
Balazy M / 2004 / Eicosanomics: targeted lipidomics of eicosanoids in biological systems / Prostaglandins Other Lipid Mediat 73:173~180
Theken KN / 2011 / Activation of the acute inflammatory response alters cytochrome P450 expression and eicosanoid metabolism / Drug Metab Dispos 39:22~29
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