지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 100 - 104 (5page)
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초록· 키워드
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Background: Malaria is a problematic disease in Korea, and microscopic examination of Giemsa-stained blood smear has been used as the gold standard for its diagnosis. However, this technique is time-consuming and has low sensitivity in samples with low numbers of malarial parasites (<20 parasites/μL). Here, we evaluated the performance characteristics of the LG Advansure^(TM) Malaria P.f./P.v. real-time QPCR (LG life sciences,Korea).
Methods: Blood samples from 173 persons who visited Korea University Ansan Hospital were evaluated. QPCR was performed in 73 malaria patients and 100 healthy subjects by using the LG Advansure Malaria P.f./P.v. real-time QPCRR kit, and the results were compared with those of microscopy.
The detection limit of this kit was determined by serial dilution of Plasmodium-infected blood with normal blood (blood not infected with Plasmodium).
Results: Among the 73 patients that were microscopically confirmed to have malaria (Plasmodium vivax infection, N=70, P. falciparum infection,N=3), 69 patients were diagnosed with P. vivax infection and 3 were diagnosed with P. falciparum infection by LG Advansure^(TM) Malaria P.f./P.v. realtime QPCR. Both the tests indicated absence of infection in the 100 healthy subjects. The detection limit of LG Advansure^(TM)Malaria P.f./P.v. real-time QPCR was 0.1 parasite/μL.
Conclusions: LG Advansure^(TM Malaria P.f./P.v. real-time QPCRis a very sensitive and specific technique and can be used as a confirmatory test for malaria.
목차
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참고문헌 (17)
참고문헌 신청
Amexo M / 2004 / Malaria misdiagnosis: effects on the poor and vulnerable / Lancet 364 : 1896 ~ 1898
Calderaro A / 2004 / Evaluation of a new plate hybridization assay for the laboratory diagnosis of imported malaria in Italy / New Microbiol 27 : 163 ~ 171
Cho YH / 2001 / Transfusion?transmitted malaria in Korea?10 cases during 1997-2001 / Korean J Blood Transfus 12 : 263 ~ 270
Farcas GA / 2004 / Evaluation of the RealArt Malaria LC real-time PCR assay for malaria diagnosis / J Clin Microbiol 42 : 636 ~ 638
Gama BE / 2007 / Real-time PCR versus conventional PCR for malaria parasite detection in low-grade parasitemia / Exp Parasitol 116 : 427 ~ 432
Calderaro A / 2004 / Evaluation of a new plate hybridization assay for the laboratory diagnosis of imported malaria in Italy / New Microbiol 27 : 163 ~ 171
Cho YH / 2001 / Transfusion?transmitted malaria in Korea?10 cases during 1997-2001 / Korean J Blood Transfus 12 : 263 ~ 270
Farcas GA / 2004 / Evaluation of the RealArt Malaria LC real-time PCR assay for malaria diagnosis / J Clin Microbiol 42 : 636 ~ 638
Gama BE / 2007 / Real-time PCR versus conventional PCR for malaria parasite detection in low-grade parasitemia / Exp Parasitol 116 : 427 ~ 432
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