지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 111 - 116 (6page)
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초록· 키워드
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We aimed to observe antimicrobial resistance patterns and integron carriage of Esche- richia coli isolates causing community-acquired infections. Two hundred sixty-eight E. coli strains were obtained from outpatients with various infections at different polyclinics at the 82nd Year of State Hospital in Rize, Turkey. Susceptibility to antimicrobials was tested us- ing a disk diffusion method. The presence of integrons was examined using PCR with specific primers. Positive PCR results were confirmed by sequencing. A broth mating method was used for conjugation assays. Extragenic palindromic-PCR was performed us- ing the oligonucleotide primer BOXA1R. Resistance frequency for ampicillin, trime- thoprim/sulfamethoxazole, and tetracycline was determined as 50.6%, 33.5%, and 36.8% respectively. No strains were resistant to amikacin. Seventy isolates were positive for the intI1 gene, of which 49 carried gene cassettes. Eleven isolates were positive for the intI2 gene, eight of which carried gene cassettes. Seven gene cassettes ( dfrA1, dfrA5, dfrA7, dfrA17, aadA1, aadA5 , and sat2 ) were predominantly harbored in integrons. We detected conjugative plasmids harboring integrons in two E. coli strains. Four strain clus- ters were yielded by BOX-PCR fingerprints showing that they were clonally related. No ap- parent relationship occurred among class 1 and 2 integron-carrying strains. We conclude that integrons are widespread in genetically variable E. coli strains and will continue to mediate dissemination of resistance genes in the community.
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참고문헌 (18)
참고문헌 신청
Jones RN / 1999 / Characteristics of pathogens causing urinary tract infections in hospitals in North America: results from the SENTRY Antimicrobial Surveillance Program, 1997 / Diagn Microbiol Infect Dis 35:55~63
Rice LB / 2009 / The clinical consequences of antimicrobial resistance / Curr Opin Microbiol 12:476~481
Cocchi S / 2007 / Distribution and characterization of integrons in Escherichia coli strains of animal and human origin / FEMS Immunol Med Microbiol 50:126~132
Sandalli C / 2010 / Prevalence of integrons and a new dfrA17 variant in Gram-negative bacilli that cause community-acquired infections / Microbiol Immun 54:164~169
National Committee for Clinical Laboratory Standards / 2000 / Performance standards for antimicrobial susceptibility testing. Tenth Informational supplement, M100-S10 (M2) / National Committee for Clinical Laboratory Standards
Rice LB / 2009 / The clinical consequences of antimicrobial resistance / Curr Opin Microbiol 12:476~481
Cocchi S / 2007 / Distribution and characterization of integrons in Escherichia coli strains of animal and human origin / FEMS Immunol Med Microbiol 50:126~132
Sandalli C / 2010 / Prevalence of integrons and a new dfrA17 variant in Gram-negative bacilli that cause community-acquired infections / Microbiol Immun 54:164~169
National Committee for Clinical Laboratory Standards / 2000 / Performance standards for antimicrobial susceptibility testing. Tenth Informational supplement, M100-S10 (M2) / National Committee for Clinical Laboratory Standards
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