지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 157 - 166 (10page)
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초록· 키워드
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Objective To observe the changes in protein expression induced by botulinum toxin A (BoNT-A) injection and to characterize the molecular and cellular action of mechanisms of BoNT-A injection on skeletal muscles using proteomic elements as biomarkers.Methods BoNT-A was injected into left gastrocnemius muscles of 12 Sprague-Dawley rats (2 months of age) at a dosage of 5 units/kg body weight. For the controls same volume of normal saline was injected to right gastrocnemius muscle of each rat. Muscle samples were obtained at 4 time points (3 rats per time point): 3, 7, 14, and 56 day post-injection. To reveal the alterations in muscle protein, we performed 2-dimensional electrophoresis (2DE) and compared Botox group and normal saline group at each time point. Altered protein spots in 2DE were identified using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer (MALDI-TOF MS) proteomics analysis.Results Compared with normal saline group, 46 protein spots showed changed protein expression. Twelve protein spots demonstrated increased volume and 34 protein spots demonstrated decreased volume. Among spots of decreased volume, 17 spots showed statistically significant differences. Thirty-eight identified proteins were associated with alterations in energy metabolism, muscle contractile function, transcription, translation, cell proliferation, and cellular stress response.Conclusion BoNT-A gives influences on muscle contractile function and energy metabolism directly or indirectly besides neurotoxic effects. Proteomic expression provides better understanding about the effect of BoNT-A on skeletal muscle.
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참고문헌 (22)
참고문헌 신청
Boutsen F. / 2002 / Botox treatment in adductor spasmodic dysphonia : a metaanalysis / J Speech Lang Hear Res 45 : 469 ~ 481
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 :
Das TK. / 1989 / Botulinum toxin in treating spasticity / Br J Clin Pract 43 : 401 ~ 403
Duchen LW. / 1971 / Changes in the electron microscopic structure of slow and fast skeletal muscle fibres of the mouse after the local injection of botulinum toxin / J Neuro
Dunne JW. / 1995 / Treatment of chronic limb spasticity with botulinum toxin A / J Neurol Neurosurg Psychiatr 58 : 232 ~ 235
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 :
Das TK. / 1989 / Botulinum toxin in treating spasticity / Br J Clin Pract 43 : 401 ~ 403
Duchen LW. / 1971 / Changes in the electron microscopic structure of slow and fast skeletal muscle fibres of the mouse after the local injection of botulinum toxin / J Neuro
Dunne JW. / 1995 / Treatment of chronic limb spasticity with botulinum toxin A / J Neurol Neurosurg Psychiatr 58 : 232 ~ 235
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