지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 165 - 173 (9page)
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초록· 키워드
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Objective To identify the effective injection route for adult human bone marrow stromal cells into traumatic brain injured rats.
Method The TBI rats were created by the lateral percussion model (HD1700, Dragonfly, Silver Spring, USA). Eight rats without stem cell transplantation were assigned to a control group. We performed adult human bone marrow stromal cell transplantation into the contralateral hemisphere (n=7), the ipsilateral brain lesion (n=8) and via a tail vein (n=11), respectively, at 24 hours after brain injury. For all of the groups, MRS (magnetic resonance spectroscopy) study, behavior tests, rotarod tests and Barnes maze tests were conducted on day 1, day 7, day 42 and day 84. Sixteen rats were randomly assigned and were sacrificed for immunohistochemical staining.
Results At day 42 (p=0.048) and day 84 (p=0.031) after TBI, the ratio of N-acetylaspartate to creatine (NAA/Cr) of the ipsilateral hemisphere was decreased in the control group, as assessed by MRS, whereas the ratio was increased in the other groups. On the post hoc analysis, significant differences were obtained among the intravenous group and the control group for the NAA/Cr ratio of the ipsilateral hemisphere at day 84 after TBI (p=0.050). However, there was no significant improvement on the behavior test, the rotarod test and the Barnes maze test. The cells were positively stained with antibodies to MAB-1281 and to GFAP.
Conclusion We confirmed that adult human bone marrow stromal cell transplantation induced an increase of the NAA/Cr ratio of the ipsilateral hemisphere at day 84 in the intravenous group. Therefore, we suggest the intravenous route is more effective for mesenchymal stem cell transplantation.
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참고문헌 (25)
참고문헌 신청
Cecil KM / 1998 / Proton magnetic resonance spectroscopy for detection of axonal injury in the splenium of the corpus callosum of brain-injured patients / J Neurosurg 88 : 7
Emsley JG / 2005 / Adult neurogenesis and repair of the adult CNS with neural progenitors, precursors, and stem cells / Prog Neurobiol 75 : 321 ~ 341
Faul M / 2004 / Traumatic brain injury in the United States. Emergency department visits, hospitalizations and deaths 2002-2006 / Centers for Disease Control and Prevention : 1 ~ 76
Fujimoto ST / 2004 / Motor and cognitive function evaluation following experimental traumatic brain injury / Neurosci Biobehav Rev 28 : 365 ~ 378
Gage FH / 2000 / Mammalian neural stem cells / Science 287 : 1433 ~ 1438
Emsley JG / 2005 / Adult neurogenesis and repair of the adult CNS with neural progenitors, precursors, and stem cells / Prog Neurobiol 75 : 321 ~ 341
Faul M / 2004 / Traumatic brain injury in the United States. Emergency department visits, hospitalizations and deaths 2002-2006 / Centers for Disease Control and Prevention : 1 ~ 76
Fujimoto ST / 2004 / Motor and cognitive function evaluation following experimental traumatic brain injury / Neurosci Biobehav Rev 28 : 365 ~ 378
Gage FH / 2000 / Mammalian neural stem cells / Science 287 : 1433 ~ 1438
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