지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 184 - 189 (6page)
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초록· 키워드
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Background: Norovirus is a leading cause of epidemic and sporadic acute gastroenteritis worldwide. Because of the rapid transmission of the virus, early detection is important to prevent outbreak of norovirus infection. To evaluate the performance of a newly introduced rapid antigen test for detecting human norovirus in stool specimens, we compared it with the established ELISA test and real time quantitative reverse transcription PCR (qRT-PCR).
Methods: One hundred and eighty-four stool samples were analyzed by rapid antigen test (Denka-Seiken, Japan), ELISA (R-Biopharm, Germany),and qRT-PCR (R-Biopharm, Germany). Overall percent agreement, percent positive agreement (PPA), and percent negative agreement (NPA) of the rapid antigen test in comparison with ELISA and qRT-PCR were obtained.
Results: Positive rates of rapid antigen test, ELISA, and qRT-PCR were 44.0% (81/184), 51.6% (95/184), and 42.9% (79/184), respectively. Seventy samples (38.0%) showed all positive, and 86 samples (46.7%) showed all negative results by three methods. Overall percent agreement of three methods was 84.8% (156/184). Overall percent agreement, PPA, and NPA of the rapid antigen test in comparison with qRT-PCR were 89.1%,88.6%, and 89.5%, respectively, and those of the rapid antigen test in comparison with ELISA were 90.2%, 83.2%, and 97.8%, respectively. Total procedure of the rapid antigen test was finished within 20 min.
Conclusions: Rapid antigen test was easier and quicker to perform, and showed high agreement rates with ELISA and qRT-PCR. This test may be useful for rapid screening of norovirus infection.
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참고문헌 (19)
참고문헌 신청
Ando T / 2000 / Genetic classification of “Norwalklike viruses” / J Infect Dis 181 : 336 ~ 348
Atmar RL / 2001 / Diagnosis of nonc‘Ltivatable gastroenteritis viruses, the human caliciviruses / Clin Microbiol Rev 14 : 15 ~ 37
Barreira DM / 2009 / Viral load and genotypes of noroviruses in symptomatic and asymptomatic children in Southeastern Brazil / J Clin Virol 47 : 60 ~ 64
Bruins MJ / 2010 / Evaluation of a rapid immunochromatographic test for the detection of norovirus in stool samples / Eur J Clin Microbiol Infect Dis 29 : 741 ~ 743
B‘Ll RA / 2006 / Emergence of a new norovirus genotype Ⅱ.4 variant associated with global outbreaks of gastroenteritis / J Clin Microbiol 44 : 327 ~ 333
Atmar RL / 2001 / Diagnosis of nonc‘Ltivatable gastroenteritis viruses, the human caliciviruses / Clin Microbiol Rev 14 : 15 ~ 37
Barreira DM / 2009 / Viral load and genotypes of noroviruses in symptomatic and asymptomatic children in Southeastern Brazil / J Clin Virol 47 : 60 ~ 64
Bruins MJ / 2010 / Evaluation of a rapid immunochromatographic test for the detection of norovirus in stool samples / Eur J Clin Microbiol Infect Dis 29 : 741 ~ 743
B‘Ll RA / 2006 / Emergence of a new norovirus genotype Ⅱ.4 variant associated with global outbreaks of gastroenteritis / J Clin Microbiol 44 : 327 ~ 333
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