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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 265 - 270 (6page)
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초록· 키워드
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Background: Members of the Acinetobacter calcoaceticus-baumannii (Acb) complex are important opportunistic bacterial pathogens and present significant therapeutic challenges in the treatment of nosocomial infections. In the present study, we investigated the integrons and various genes involved in resistance to carbapenems, aminoglycosides, and fluoroquinolones in 56 imipenem-nonsusceptible Acb complex isolates.
Methods: This study included 44 imipenem-nonsusceptible A. baumannii, 10 Acinetobacter genomic species 3, and 2 Acinetobacter genomic species 13TU strains isolated in Daejeon, Korea. The minimum inhibitory concentrations (MICs) were determined by Etest. PCR and DNA sequencing were used to identify the genes that potentially contribute to each resistance phenotype.
Results: All A. baumannii isolates harbored the blaOXA-51-like gene, and 21 isolates (47.7%) co-produced OXA-23. However, isolates of Acinetobacter genomic species 3 and 13TU only contained blaIMP-1 or blaVIM-2. Most Acb complex isolates (94.6%) harbored class 1 integrons, armA, and/or aminoglycoside-modifying enzymes (AMEs). Of particular note was the fact that armA and aph(3´)-Ia were only detected in A. baumannii isolates, which were highly resistant to amikacin (MIC50≥256) and gentamicin (MIC50≥1,024). In all 44 A. baumannii isolates, resistance to fluoroquinolones was conferred by sense mutations in the gyrA and parC. However, sense mutations in parC were not found in Acinetobacter genomic species 3 or 13TU isolates.
Conclusions: Several differences in carbapenem, aminoglycoside, and fluoroquinolone resistance gene content were detected among Acb complex isolates. However, most Acb complex isolates (87.5%) possessed integrons, carbapenemases, AMEs, and mutations in gyrA. The co-occurrence of several resistance determinants may present a significant threat.
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참고문헌 (28)
참고문헌 신청
Lee K / 2009 / Wide dissemination of OXA-type carbapenemases in clinical Acinetobacter spp. isolates from South Korea / International Journal Of Antimicrobial Agents 33(6):520~524
Turton JF / 2006 / The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii / FEMS Microbiol Lett 258:72~77
성지연 / 2008 / IMP-1과 OXA -lactamase를 생성하는 Carbapenem 내성 Acinetobacter baumanni 의 확산 / Annals of Laboratory Medicine 28(1):16~23
Yum JH / 2002 / Molecular characterization of metallo-β-lactamase-producing Acinetobacter baumannii and Acinetobacter genomospecies 3 from Korea: identification of two new integrons carrying the bla(VIM-2) gene cassettes / J Antimicrob Chemother 49:837~840
Zhou Y / 2010 / Distribution of 16S rRNA methylases among different species of Gram-negative bacilli with high-level resistance to aminoglycosides / Eur J Clin Microbiol Infect Dis 29:1349~1353
Turton JF / 2006 / The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii / FEMS Microbiol Lett 258:72~77
성지연 / 2008 / IMP-1과 OXA -lactamase를 생성하는 Carbapenem 내성 Acinetobacter baumanni 의 확산 / Annals of Laboratory Medicine 28(1):16~23
Yum JH / 2002 / Molecular characterization of metallo-β-lactamase-producing Acinetobacter baumannii and Acinetobacter genomospecies 3 from Korea: identification of two new integrons carrying the bla(VIM-2) gene cassettes / J Antimicrob Chemother 49:837~840
Zhou Y / 2010 / Distribution of 16S rRNA methylases among different species of Gram-negative bacilli with high-level resistance to aminoglycosides / Eur J Clin Microbiol Infect Dis 29:1349~1353
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