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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 302 - 306 (5page)
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Loss of heterozygosity (LOH) in chromosome 6p has been reported in a number of tumors and some hematologic malignancies, including ALL. LOH in chromosome 6p, on which the HLA genes are located, can give rise to false homozygosity results in HLA genotyping of patients with hematologic malignancies. Here we report false homozygosity results in HLA genotyping due to the loss of whole chromosome 6 in the neoplastic cells of a patient with ALL. A 33-yr-old Korean female patient was admitted for the evaluation of leukocytosis detected during a workup for headache. Her initial white blood cell count was 336.9×10^9/L with 84% of blasts in the differential count. Precursor-B lymphoblastic leukemia was diagnosed from a subsequent bone marrow study. HLA high-resolution genotyping of the patient was requested at the time of diagnosis for possible hematopoietic stem cell transplantation. Homozygosity results (A*02:01, B*54:01, C*08:01, DQB1*04:01) were obtained, except for the DRB1 locus (DRB1*04:05, DRB1*11:01), in sequence-based typing. Conventional karyotyping of bone marrow metaphase cells revealed chromosomal abnormalities, with loss of multiple chromosomes including chromosome 6, and reduplication of the remaining chromosomes: 29,X,+X,+8,inv(9)(p11q13),+10,+14,+18,+21[15]/58,idemX2[3]/46,XX,inv(9)[2]. LOH at the HLA region was suspected and HLA genotyping was repeated with the peripheral blood in remission state after induction chemotherapy. All 5 HLA loci were typed as heterozygous (A*02:01, A*02:06, B*40:01, B*54:01, C*03:04, C*08:01, DRB1*04:05, DRB1*11:01, DQB1*03:01, DQB1*04:01). To avoid false HLA typing results in patients with hematologic malignancies, clinicians, as well as laboratory personnel, need to be aware of such problems and take appropriate precautions.
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참고문헌 (21)
참고문헌 신청
Baccichet A / 1997 / Allelic loss in childhood acute lymphoblastic leukemia / Leuk Res 21 : 817 ~ 823
Browning M / 1996 / Mechanisms of loss of HLA class I expression on colorectal tumor cells / Tissue Antigens 47 : 364 ~ 371
Browning MJ / 1993 / Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy / J Immunother Emphasis
Coppage M / 2010 / Leukemia specific loss of heterozygosity at MHC locus detected at confirmatory typing of HSCT recipient with CLL / Hum Immunol 71 : 130 ~
D’Urso CM / 1991 / Lack of HLA class I antigen expression by cultured melanoma cells FO-1 due to a defect in B2m gene expression / J Clin Invest 87 : 284 ~ 292
Browning M / 1996 / Mechanisms of loss of HLA class I expression on colorectal tumor cells / Tissue Antigens 47 : 364 ~ 371
Browning MJ / 1993 / Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy / J Immunother Emphasis
Coppage M / 2010 / Leukemia specific loss of heterozygosity at MHC locus detected at confirmatory typing of HSCT recipient with CLL / Hum Immunol 71 : 130 ~
D’Urso CM / 1991 / Lack of HLA class I antigen expression by cultured melanoma cells FO-1 due to a defect in B2m gene expression / J Clin Invest 87 : 284 ~ 292
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