지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 264 - 268 (5page)
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초록· 키워드
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We present here occurence of PCR-positive but culture-negative for vanB vancomycin-resistant enterococci (VRE) from an enrichment broth of a stool surveillance culture in a patient suffering from Parkinson’s disease, who was transferred from a long-term care facility because of aspiration pneumonia. He developed VRE bacteriuria at the hospital day 42. vanA and vanB genes were detected from 6 μg/mL vancomycin-containing BBL Enterococcosel broth (BD), of which color changed to black after overnight incubation, by both Seeplex VRE detection (Seegene, Seoul, Korea) and Anyplex VanR real-time PCR (Seegene). Subculture of an aliquot of the blackened broth on blood agar plate produced only vanA VRE. All of the four subsequent consecutive surveillance cultures for 1 month until discharge at hospital day 75 resulted in PCR-positive but culture-negative for vanB VRE from the enrichment broths. Therefore, the presence of a non-enterococcal intrinsic reservoir bearing vanB is more likely than low burden of vanB VRE. Considering the rare occurrence of vanB VRE in Korea, vanB-positive PCR results from the enrichment broth requires confirmation by microbiological studies.
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참고문헌 (20)
참고문헌 신청
Ballard SA / 2005 / Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci:
Ballard SA / 2005 / Molecular characterization of vanB elements in naturally occurring gut anaerobes / Antimicrob Agents Chemother 49 : 1688 ~ 1694
Cheong HJ / 2002 / Clinical features of VRE infection, microbiologic characteristics and genetic relatedness of VRE isolates from six university hospitals / Korean J Infect D
Domingo MC / 2005 / Characterization of a Tn5382-like transposon containing the vanB2 gene cluster in a Clostridium strain isolated from human faeces / J Antimicrob Chemothe
Kee SY / 2012 / Healthcareassociated risk factors of vancomycin-resistant Enterococci colonization among outpatients undergoing hemodialysis / Jpn J Infect Dis 65 : 57 ~ 60
Ballard SA / 2005 / Molecular characterization of vanB elements in naturally occurring gut anaerobes / Antimicrob Agents Chemother 49 : 1688 ~ 1694
Cheong HJ / 2002 / Clinical features of VRE infection, microbiologic characteristics and genetic relatedness of VRE isolates from six university hospitals / Korean J Infect D
Domingo MC / 2005 / Characterization of a Tn5382-like transposon containing the vanB2 gene cluster in a Clostridium strain isolated from human faeces / J Antimicrob Chemothe
Kee SY / 2012 / Healthcareassociated risk factors of vancomycin-resistant Enterococci colonization among outpatients undergoing hemodialysis / Jpn J Infect Dis 65 : 57 ~ 60
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