지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 408 - 410 (3page)
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초록· 키워드
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Anti-donor HLA-specific antibodies (DSA) are associated with poor graft outcomes in renal transplantations [1, 2]. Panel reac- tive antibody (PRA) assays using the Luminex platform are commonly used to detect DSA. PRA assays are performed by using three different panels: 1) pooled antigen panels that use bead populations coated in affinity-purified HLA molecules ob- tained from multiple cell lines, which are used as screening tests; 2) phenotype panels that use bead populations bearing HLA proteins from a cell line derived from a single individual; and 3) single-antigen bead (SAB) assays that use beads coated in molecules representing a single cloned allelic HLA antigen [3]. Each bead population in phenotype panels features more than one type of HLA molecule, thus requiring expertise to inter- pret the results, whereas SAB assays provide accurate identifi- cation of HLA antibodies [3]. However, cloned HLA antigens are not in their native state. Purification and bead coating can lead to conformational changes that could cause binding of clinically irrelevant antibodies [4-9]. Here, we report a case with sus- pected false reactions against denatured HLA class II molecules in SAB assays.
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참고문헌 (11)
참고문헌 신청
El-Awar N / 2009 / Epitopes of human leukocyte antigen class I antibodies found in sera of normal healthy males and cord blood / Hum Immunol 70 : 844 ~ 853
Grenzi PC / 2013 / Antibodies against denatured HLA class II molecules detected in luminex-single antigen assay / Hum Immunol 74 : 1300 ~ 1303
Jacob EK / 2011 / Positive virtual crossmatch with negative flow crossmatch results in two cases / Transpl Immunol 25 : 77 ~ 81
Kim SY / 2012 / Evaluation of flow cytometric crossmatch results in comparison with donor-specific antibodies detected by Luminex-PRA tests in organ transplantation patients
Loupy A / 2012 / The impact of donor-specific anti-HLA antibodies on late kidney allograft failure / Nat Rev Nephrol 8 : 348 ~ 357
Grenzi PC / 2013 / Antibodies against denatured HLA class II molecules detected in luminex-single antigen assay / Hum Immunol 74 : 1300 ~ 1303
Jacob EK / 2011 / Positive virtual crossmatch with negative flow crossmatch results in two cases / Transpl Immunol 25 : 77 ~ 81
Kim SY / 2012 / Evaluation of flow cytometric crossmatch results in comparison with donor-specific antibodies detected by Luminex-PRA tests in organ transplantation patients
Loupy A / 2012 / The impact of donor-specific anti-HLA antibodies on late kidney allograft failure / Nat Rev Nephrol 8 : 348 ~ 357
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