지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 201 - 205 (5page)
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초록· 키워드
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Background: We evaluated the performance of various commercial assays for the molecular detection of human papillomavirus (HPV); the recently developed AdvanSure HPV Screening real-time PCR assay (AdvanSure PCR) and the Abbott RealTime High Risk HPV PCR assay (Abbott PCR) were compared with the Hybrid Capture 2 HPV DNA Test (HC2).
Methods: All 3 tests were performed on 177 samples, and any sample that showed a discrepancy in any of the 3 tests was genotyped using INNO-LiPA HPV genotyping and/or sequencing. On the basis of these results, we obtained a consensus HPV result, and the performance of each test was evaluated. We also evaluated high-risk HPV 16/18 detection by using the 2 real-time PCR assays.
Results: Among the 177 samples, 65 were negative and 75 were positive in all 3 assays; however, the results of the 3 assays with 37 samples were discrepant. Compared with the consensus HPV result, the sensitivities and specificities of HC2, AdvanSure PCR, and Abbott PCR were 97.6%, 91.7%, and 86.9% and 83.9%, 98.8%, and 100.0%, respectively. For HPV type 16/18 detection, the concordance rate between the AdvanSure PCR and Abbott PCR assays was 98.3%; however, 3 samples were discrepant (positive in AdvanSure PCR and negative in Abbott PCR) and were confirmed as HPV type 16 by INNO-LiPA genotyping and/or sequencing.
Conclusions: For HPV detection, the AdvanSure HPV Screening real-time PCR assay and the Abbott PCR assay are less sensitive but more specific than the HC2 assay, but can simultaneously differentiate type 16/18 HPV from other types.
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참고문헌 (16)
참고문헌 신청
/ 2010 / Instructor’s insert of Abbott RealTime High Risk HPV / Abbott molecular
/ 2010 / Instructor’s insert of AdvanSure HPV Screening real-time PCR / LG Life Science
Arbyn M / 2009 / How to evaluate emerging technologies in cervical cancer screening? / Int J Cancer 125 : 2489 ~ 2496
Castle PE / 2008 / Human papillomavirus genotype specificity of hybrid capture 2 / J Clin Microbiol 46 : 2595 ~ 2604
Cuzick J / 2006 / Overview of the European and North American studies on HPV testing in primary cervical cancer screening / Int J Cancer 119 : 1095 ~ 1101
/ 2010 / Instructor’s insert of AdvanSure HPV Screening real-time PCR / LG Life Science
Arbyn M / 2009 / How to evaluate emerging technologies in cervical cancer screening? / Int J Cancer 125 : 2489 ~ 2496
Castle PE / 2008 / Human papillomavirus genotype specificity of hybrid capture 2 / J Clin Microbiol 46 : 2595 ~ 2604
Cuzick J / 2006 / Overview of the European and North American studies on HPV testing in primary cervical cancer screening / Int J Cancer 119 : 1095 ~ 1101
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