지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 126 - 132 (7page)
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초록· 키워드
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Background: The identification of molds in clinical laboratories is largely on the basis of phenotypic criteria, the classification of which can be subjective. Recently, molecular methods have been introduced for identification of pathogenic molds in clinical settings. Here, we employed comparative sequence analysis to identify molds.
Methods: A total of 47 clinical mold isolates were used in this study, including Aspergillus and Trichophyton. All isolates were identified by phenotypic properties, such as growth rate, colony morphology, and reproductive structures. PCR and direct sequencing, targeting the internal transcribed spacer (ITS) region, the D1/D2 region of the 28S subunit, and the ß-tubulin gene, were performed using primers described previously. Comparative sequence analysis by using the GenBank database was performed with the basic local alignment search tool (BLAST) algorithm.
Results: For Aspergillus, 56% and 67% of the isolates were identified to the species level by using ITS and ß-tubulin analysis, respectively. Only D1/D2 analysis was useful for Trichophyton identification, with 100% of isolates being identified to the species level. Performances of ITS and D1/D2 analyses were comparable for species-level identification of molds other than Aspergillus and Trichophyton. In contrast, the efficacy of ß-tubulin analysis was limited to genus identification because of the paucity of database information for this gene.
Conclusions: The molecular methods employed in this study were valuable for mold identification, although the different loci used had variable usefulness, according to mold genus. Thus, a tailored approach is recommended when selecting amplification targets for molecular identification of molds.
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참고문헌 (19)
참고문헌 신청
Atkins SD / 2004 / Fungal molecular diagnostics: a mini review / J Appl Genet 45 : 3 ~ 15
Balajee SA / 2005 / Aspergillus lentulus sp. nov., a new sibling species of A. fumigatus / Eukaryot Cell 4 : 625 ~ 632
Balajee SA / 2009 / Sequence-based identification of Aspergillus, fusarium, and mucorales species in the clinical mycology laboratory: where are we and where should we go fro
Barton RC / 2010 / Isolation of the fungus Geosmithia argillacea in sputum of people with cystic fibrosis / J Clin Microbiol 48 : 2615 ~ 2617
Ciardo DE / 2007 / Identification of moulds in the diagnostic laboratory--an algorithm implementing molecular and phenotypic methods / Diagn Microbiol Infect Dis 59 : 49 ~ 60
Balajee SA / 2005 / Aspergillus lentulus sp. nov., a new sibling species of A. fumigatus / Eukaryot Cell 4 : 625 ~ 632
Balajee SA / 2009 / Sequence-based identification of Aspergillus, fusarium, and mucorales species in the clinical mycology laboratory: where are we and where should we go fro
Barton RC / 2010 / Isolation of the fungus Geosmithia argillacea in sputum of people with cystic fibrosis / J Clin Microbiol 48 : 2615 ~ 2617
Ciardo DE / 2007 / Identification of moulds in the diagnostic laboratory--an algorithm implementing molecular and phenotypic methods / Diagn Microbiol Infect Dis 59 : 49 ~ 60
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