지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 28 - 34 (7page)
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초록· 키워드
오류제보하기
Background: The usefulness of the CytoDiff flow cytometric system (Beckman Coulter, USA) has been studied in various conditions, but its performance including rapidity in detecting and counting blasts, the most significant abnormal cells in the peripheral blood, has not been well evaluated. The objective of this study was to evaluate the performance of the CytoDiff differential counting method in challenging samples with blasts.
Methods: In total, 815 blood samples were analyzed. Samples flagged as “blasts” or “variant lymphocytes” and showing <10% blasts by manual counts were included. In total, 322 samples showed blasts on manual counts, ranging from 0.5% to 99%. The CytoDiff method was performed by flow cytometry (FC500; Beckman Coulter, USA) with a pre-mixed CytoDiff reagent and analyzing software (CytoDiff CXP 2.0; Beckman Coulter).
Results: The average time required to analyze 20 samples was approximately 60 min for manual counts, and the hands-on time for the CytoDiff method was 15 min. The correlation between the CytoDiff and manual counts was good (r>0.8) for neutrophils and lymphocytes but poor (r<0.8) for other cells. When the cutoff value of the CytoDiff blast count was set at 1%, the sensitivity was 94.4% (95% CI; 91.2-96.6) and specificity was 91.9% (95% CI; 89.0-94.1). The positive predictive value was 88.4% (95% CI; 84.4-91.5) (304/344 cases) and negative predictive value was 96.2% (95% CI; 93.9-97.7) (453/471 cases). The CytoDiff blast counts correlated well to the manual counts (r=0.9223).
Conclusions: The CytoDiff method is a specific, sensitive, and rapid method for counting blasts. A cutoff value of 1% of at least 1 type of blast is recommended for positive CytoDiff blast counts.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Barnes PW / 2005 / international consensus group for hematology. The international consensus groupfor hematology review: suggested criteria for action following automated CBC
Briggs C / 2009 / Can automated blood film analysis replace the manual differential? Anevaluation of the CellaVision DM96 automated image analysis system / Int J LabHematol 3
Eilertsen H / 2013 / The usefulness of blast flags on the Sysmex XE-5000 is questionable / Am J Clin Pathol 139 : 633 ~ 640
Faucher JL / 2007 / “6 markers/5 colors” extended white blood cell differential by flow cytometry / Cytometry A 71 : 934 ~ 944
Fuentes-Arderiu X / 2009 / Measurement uncertainty in manual differential leukocyte counting / Clin Chem Lab Med 47 : 112 ~ 115
Briggs C / 2009 / Can automated blood film analysis replace the manual differential? Anevaluation of the CellaVision DM96 automated image analysis system / Int J LabHematol 3
Eilertsen H / 2013 / The usefulness of blast flags on the Sysmex XE-5000 is questionable / Am J Clin Pathol 139 : 633 ~ 640
Faucher JL / 2007 / “6 markers/5 colors” extended white blood cell differential by flow cytometry / Cytometry A 71 : 934 ~ 944
Fuentes-Arderiu X / 2009 / Measurement uncertainty in manual differential leukocyte counting / Clin Chem Lab Med 47 : 112 ~ 115
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