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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 159 - 166 (8page)
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Background: N-ras mutations are one of the most commonly detected abnormalities of myeloid origin. N-ras mutations result in a constitutively active N-ras protein that induces uncontrolled cell proliferation and inhibits apoptosis. We analyzed N-ras mutations in adult patients with AML at a particular institution and compared pyrosequencing analysis with a direct sequencing method for the detection of N-ras mutations.
Methods: We analyzed 90 bone marrow samples from 83 AML patients. We detected Nras mutations in codons 12, 13, and 61 using the pyrosequencing method and subsequently confirmed all data by direct sequencing. Using these methods, we screened the N-ras mutation quantitatively and determined the incidence and characteristic of N-ras mutation.
Results: The incidence of N-ras mutation was 7.2% in adult AML patients. The patients with N-ras mutations showed significant higher hemoglobin levels (P =0.022) and an increased incidence of FLT3 mutations (P =0.003). We observed 3 cases with N-ras mutations in codon 12 (3.6%), 2 cases in codon 13 (2.4%), and 1 case in codon 61 (1.2%). All the mutations disappeared during chemotherapy.
Conclusions: There is a low incidence (7.2%) of N-ras mutations in AML patients compared with other populations. Similar data is obtained by both pyrosequencing and direct sequencing. This study showed the correlation between the N-ras mutation and the therapeutic response. However, pyrosequencing provides quantitative data and is useful for monitoring therapeutic responses.
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참고문헌 (24)
참고문헌 신청
Ahmadian A / 2006 / Pyrosequencing: history, biochemistry and future / Clin Chim Acta 363 : 83 ~ 94
Auewarakul CU / 2006 / Frequency of RAS gene mutation and its cooperative genetic events in Southeast Asian adult acute myeloid leukemia / Eur J Haematol 77 : 51 ~ 56
Bacher U / 2006 / Implications of NRAS mutations in AML: a study of 2502 patients / Blood 107 : 3847 ~ 3853
Bowen DT / 2005 / RAS mutation in acute myeloid leukemia is associated with distinct cytogenetic subgroups but does not influence outcome in patients younger than 60 years /
Darley RL / 1999 / Mutant RAS inhibits neutrophil but not macrophage differentiation and allows continued growth of neutrophil precursors / Exp Hematol 27 : 1599 ~ 1608
Auewarakul CU / 2006 / Frequency of RAS gene mutation and its cooperative genetic events in Southeast Asian adult acute myeloid leukemia / Eur J Haematol 77 : 51 ~ 56
Bacher U / 2006 / Implications of NRAS mutations in AML: a study of 2502 patients / Blood 107 : 3847 ~ 3853
Bowen DT / 2005 / RAS mutation in acute myeloid leukemia is associated with distinct cytogenetic subgroups but does not influence outcome in patients younger than 60 years /
Darley RL / 1999 / Mutant RAS inhibits neutrophil but not macrophage differentiation and allows continued growth of neutrophil precursors / Exp Hematol 27 : 1599 ~ 1608
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