지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 420 - 425 (6page)
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초록· 키워드
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Background: Treatment for chronic hepatitis B aims to suppress virus replication and virus sequestration in hepatocytes. Covalently closed circular (ccc) DNA is the template for transcription of viral genes and is responsible for viral persistence. However, limited data are available for quantification of hepatitis B surface antigen (HBsAg) in Korea.
Methods: We evaluated the Elecsys HBsAg II quant assay (Roche Diagnostics, USA) for within-run, between-run, and between-day precisions, linearity, carryover, and clinical specificity. In total, 156 serum samples were evaluated for correlation between HBsAg and hepatitis B virus (HBV) DNA. Serial samples were obtained from 10 patients at 0, 12, 24, 48, 72, and 96 weeks during follow-up.
Results: The assay detected HBsAg in a linear range of 0.5-48,696 IU/mL. Within-run, between- run, and between-day CVs were 2.9-4.1%, 0-1.5%, and 1.5-4.9%, respectively. Cross-reactivity between potentially interfering substances was absent, and the carryover rate was 0.00002%. The correlation of measurements between the Elecsys assay and HBV DNA PCR was weak (r=0.438, P =0.002). For predicting virologic response, cutoff values of 10,275 IU/mL and 3,846 IU/mL at 12 and 24 weeks after treatment initiation showed positive predictive values of 77.1% and 85% and negative predictive values of 84.6% and 50%, respectively.
Conclusions: The Elecsys HBsAg II quant assay showed good performance for precision, linearity, carryover rate, and specificity. HBsAg level at baseline, 12 weeks, and 24 weeks after treatment initiation can predict virologic response, and the assay can be used for HBsAg quantification in clinical practice.
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참고문헌 (22)
참고문헌 신청
Chan HL / 2007 / Serum hepatitis B surface antigen quantitation can reflect hepatitis B virus in the liver and predict treatment response / Clin Gastroenterol Hepatol 5 : 14
Chan HL / 2011 / Hepatitis B surface antigen quantification: why and how to use it in 2011-a core group report / J Hepatol 55 : 1121 ~ 1131
Chen J / 2009 / Serum HBsAg changes in HBeAg positive chronic hepatitis B patients with continuous viral load reductions during treatment with adefovir or peg-interferon-alp
Deguchi M / 2004 / Quantitation of hepatitis B surface antigen by an automated chemiluminescent microparticle immunoassay / J Virol Methods 115 : 217 ~ 222
Glass DC / 2011 / Estimating mean exposures from censored data: exposure to benzene in the Australian petroleum industry / Ann Occup Hyg 45 : 275 ~ 282
Chan HL / 2011 / Hepatitis B surface antigen quantification: why and how to use it in 2011-a core group report / J Hepatol 55 : 1121 ~ 1131
Chen J / 2009 / Serum HBsAg changes in HBeAg positive chronic hepatitis B patients with continuous viral load reductions during treatment with adefovir or peg-interferon-alp
Deguchi M / 2004 / Quantitation of hepatitis B surface antigen by an automated chemiluminescent microparticle immunoassay / J Virol Methods 115 : 217 ~ 222
Glass DC / 2011 / Estimating mean exposures from censored data: exposure to benzene in the Australian petroleum industry / Ann Occup Hyg 45 : 275 ~ 282
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