지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 138 - 142 (5page)
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초록· 키워드
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Background: Prevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia.
Methods: Single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting (HRM) analysis were used for the identification of α-thalassemia-1 Southeast Asian (SEA) and Thai type deletions and β-thalassemia 3.5-kb gene deletion. The results were compared with those obtained using conventional gap-PCR. DNA samples were derived from 28 normal individuals, 11 individuals with α-thalassemia-1 SEA type deletion, 2 with α-thalassemia-1 Thai type deletion, and 2 with heterozygous β-thalassemia 3.5-kb gene deletion.
Results: HRM analysis indicated that the amplified fragments from α-thalassemia-1 SEA type deletion, α-thalassemia-1 Thai type deletion, β-thalassemia 3.5-kb gene deletion, and the wild-type β-globin gene had specific peak heights at mean melting temperature (T_m) values of 86.89˚C, 85.66˚C, 77.24˚C, and 74.92˚C, respectively. The results obtained using single-tube multiplex real-time PCR with SYBR Green1 and HRM analysis showed 100% consistency with those obtained using conventional gap-PCR.
Conclusions: Single-tube multiplex real-time PCR with SYBR Green1 and HRM analysis is a potential alternative for routine clinical screening of the common types of α- and β-thalassemia large gene deletions, since it is simple, cost-effective, and highly accurate.
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참고문헌 (25)
참고문헌 신청
Chanprapaph P / 2002 / Prenatal diagnosis of alpha-thalassemia-1 (SEA type) by chorionic villus sampling / J Med Assoc Thai 85 : 1049 ~ 1053
Craig JE / 1992 / Molecular characterization of a novel 10.3 kb deletion causing beta-thalassaemia with unusually high Hb A2 / Br J Haematol 82 : 735 ~ 744
Eng B / 2000 / PCR-based diagnosis of the Filipino (--[FIL]) and Thai (--[THAI]) α-thalassemia-1 deletions / Am J Hematol 63 : 54 ~ 56
Fischel-Ghodsian N / 1988 / Characterization of two deletions that remove the entire human zeta-alpha globin gene complex (- -THAI and - -FIL) / Br J Haematol 70 : 233 ~ 238
Fucharoen G / 1995 / Molecular basis of alpha (0)-thalassemia in northeast of Thailand / Southeast Asian J Trop Med Public Health 26 (1) : 249 ~ 251
Craig JE / 1992 / Molecular characterization of a novel 10.3 kb deletion causing beta-thalassaemia with unusually high Hb A2 / Br J Haematol 82 : 735 ~ 744
Eng B / 2000 / PCR-based diagnosis of the Filipino (--[FIL]) and Thai (--[THAI]) α-thalassemia-1 deletions / Am J Hematol 63 : 54 ~ 56
Fischel-Ghodsian N / 1988 / Characterization of two deletions that remove the entire human zeta-alpha globin gene complex (- -THAI and - -FIL) / Br J Haematol 70 : 233 ~ 238
Fucharoen G / 1995 / Molecular basis of alpha (0)-thalassemia in northeast of Thailand / Southeast Asian J Trop Med Public Health 26 (1) : 249 ~ 251
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