지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 300 - 306 (7page)
이용수
초록· 키워드
오류제보하기
Background: Detection methods for ABO antibody (Ab) titers vary across laboratories, and the results are different depending on the method used. We aimed to compare titer values using different detection methods for the measurement of ABO Ab titers.
Methods: For ABO Ab detection, pooled group A or B red blood cells (RBCs) were reacted with each of 20 sera from blood groups A, B, or O without dithiothreitol treatment. The room-temperature (RT) incubation technique and the indirect antiglobulin test (IAT) were used in the tube test and gel card test. Flow cytometry (FCM) was performed by using anti-IgM and anti-IgG Abs.
Results: Regardless of the blood groups tested, the FCM assay with anti-IgM showed the highest titer compared to the tube test and gel card test with RT incubation in both. The tube test with IAT showed a higher titer than the gel card test with IAT (Gel-IAT) or FCM with anti-IgG in blood group A and B, while Gel-IAT showed the highest titer relative to the other tests, only for the anti-A Ab in blood group O.
Conclusions: There were significant differences in the titers depending on the detection method used, and each method showed a different detection capacity for each ABO Ab depending on the ABO blood group tested. Therefore, caution should be exercised in in- terpreting ABO Ab titer results, taking into consideration the detection method used and the blood group.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
AuBuchon JP / 2008 / Reducing the variation in performance of antibody titrations / Vox Sang 95 : 57 ~ 65
Berneman ZN / 1991 / Flow-cytometric analysis of erythrocytic blood group A antigen density profile / Vox Sang 61 : 265 ~ 274
Knight RC / 1978 / Measuring IgG anti-A/B titres using dithiothreitol (DTT) / J Clin Pathol 31 : 283 ~ 287
Kobayashi T / 2006 / A series of surveys on assay for anti-A/B antibody by Japanese ABO-incompatible Transplantation Committee / Xenotransplantation 13 : 136 ~ 140
Kumlien G / 2007 / Comparing the tube and gel techniques for ABO antibody titration, as performed in three European centers / Transplantation 84 (12) : 17 ~ 19
Berneman ZN / 1991 / Flow-cytometric analysis of erythrocytic blood group A antigen density profile / Vox Sang 61 : 265 ~ 274
Knight RC / 1978 / Measuring IgG anti-A/B titres using dithiothreitol (DTT) / J Clin Pathol 31 : 283 ~ 287
Kobayashi T / 2006 / A series of surveys on assay for anti-A/B antibody by Japanese ABO-incompatible Transplantation Committee / Xenotransplantation 13 : 136 ~ 140
Kumlien G / 2007 / Comparing the tube and gel techniques for ABO antibody titration, as performed in three European centers / Transplantation 84 (12) : 17 ~ 19
이 논문의 저자 정보
최근 본 자료
댓글(0)
0