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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 26 - 30 (5page)
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초록· 키워드
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Background: Early laboratory detection of Mycobacterium tuberculosis is crucial for con- trolling tuberculosis. We developed a hydrogel mycobacterial culture method that retains the advantages of both solid and liquid methods in terms of speed, cost, and efficiency.
Methods: Mycobacterium bovis bacillus Calmette-Guérin (BCG) suspensions and 200 acid-fast bacilli (AFB)-positive clinical specimens were inoculated in Middlebrook 7H9 liq- uid media (Becton-Dickinson and Company, USA) and mixed with 75 μL of 9-fluorenyl- methoxycarbonyl (Fmoc)-Phe-Phe-OH hydrogel stock solution in an Eppendorf tube just before culture incubation. The mixtures were cultured at 37°C for as long as 14 days to monitor culture status.
Results: The number of M. bovis BCG increased with time. For 200 AFB smear-positive specimens, 155 of 158 conventional culture-positive specimens and 4 culture-negative or contaminated specimens yielded positive cultures within 14 days. For 128 specimens positive with the liquid culture method, the time to positive culture using the hydrogel method (mean, 12.6 days; range, 7 to 14 days) was significantly shorter than that for con- ventional liquid culture (mean, 16.2 days; range, 6 to 31 days; P <0.0001).
Conclusions: The hydrogel scaffold culture system is useful for timely, economical, and efficient detection of mycobacteria in clinical specimens.
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참고문헌 (12)
참고문헌 신청
Bollela VR / 1999 / McFarland nephelometer as a simple method to estimate the sensitivity of the polymerase chain reaction using Mycobacterium tuberculosis as a research tool
Chang CL / 2001 / Survey on changes in mycobacterial testing practices in Korean Laboratories / Korean J Clin Microbiol 4 : 108 ~ 114
Jayawarna V / 2006 / Nanostructured hydrogels for three-dimensional cell culture through self-assembly of fluorenylmethoxycarbonyl-dipeptides / Adv Mater 18 : 611 ~ 614
Jayawarna V / 2007 / Three-dimensional cell culture of chondrocytes on modified di-phenylalanine scaffolds / Biochem Soc Trans 35 (Pt 3) : 535 ~ 537
Levenberg S / 2003 / Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds / Proc Natl Acad Sci USA 100 : 12741 ~ 12746
Chang CL / 2001 / Survey on changes in mycobacterial testing practices in Korean Laboratories / Korean J Clin Microbiol 4 : 108 ~ 114
Jayawarna V / 2006 / Nanostructured hydrogels for three-dimensional cell culture through self-assembly of fluorenylmethoxycarbonyl-dipeptides / Adv Mater 18 : 611 ~ 614
Jayawarna V / 2007 / Three-dimensional cell culture of chondrocytes on modified di-phenylalanine scaffolds / Biochem Soc Trans 35 (Pt 3) : 535 ~ 537
Levenberg S / 2003 / Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds / Proc Natl Acad Sci USA 100 : 12741 ~ 12746
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