지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The 'Dielectrophoretic Tweezers(DEP Tweezers)' can be used as a facile, economical toolkit for quantitative measurement of chemical and biological binding forces related to many biological interactions within a microfluidic device. Our experimental setup can probe the interaction between a single receptor molecule and its specific ligand.
Immunoglobulin G(IgG) functionalized on polystyrene microspheres has been used to detect individual surface linked Staphylococcus protein A(SpA) molecules and to characterize the strength of the noncovalent IgG-SpA bond. It was measured and compared with the existing measurements. Measured single binding force of between Goat, Rabbit IgG and SpA were 17 ± 7pN, 74 ± 16pN. This work can be used to investigate several different ligand-receptor interactions and antigen-antibody interactions.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Ahmed Touhami / / Molecular Recognition Forces between Immunoglobulin G and a Surface Protein Adhesin on Living Staphylococcus aureus / Langmuir 23 : 2755 ~ 2760
Andrea L. Stout / 2001 / Detection and Characterization of Individual Intermolecular Bonds UsingOptical Tweezers / Biophys. J 80 : 2976 ~ 2986
Cozens-Roberts, C. / 1990 / Receptormediated adhesion phenomena : model studies with the radial-flowdetachment assay / Biophys. J 58 : 107 ~ 125
D.A. Simson / 1999 / Statistical Breakage of Single Protein A-IgG Bonds Reveals Crossover from Spontaneous to Force-Induced Bond Dissociation / Phys Rev Lett 83 (3) : 652 ~ 6
George M. Whitesides / 2006 / The origins and the future of microfluidics / Nature 442
Andrea L. Stout / 2001 / Detection and Characterization of Individual Intermolecular Bonds UsingOptical Tweezers / Biophys. J 80 : 2976 ~ 2986
Cozens-Roberts, C. / 1990 / Receptormediated adhesion phenomena : model studies with the radial-flowdetachment assay / Biophys. J 58 : 107 ~ 125
D.A. Simson / 1999 / Statistical Breakage of Single Protein A-IgG Bonds Reveals Crossover from Spontaneous to Force-Induced Bond Dissociation / Phys Rev Lett 83 (3) : 652 ~ 6
George M. Whitesides / 2006 / The origins and the future of microfluidics / Nature 442
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