지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 179 - 186 (8page)
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Purpose Although the incidence of microsatellite instability (MSI) accounts for 10-15% of cases of colorectal cancer, its clinical application for all colorectal cancers has widened. We attempted to identify clinical and pathological parameters that may be helpful in selection of patients with MSI-high (MSI-H).
Materials and Methods A total of 120 resected colorectal cancers were enrolled retrospectively for this MSI study.
Polymerase chain reaction (PCR) and denaturing high performance liquid chromatography and/or real time PCR methods with five markers and immunohistochemistry (IHC) for MLH1 and MSH2were performed for analysis of cancer and blood specimens. Clinico-pathologic parameters,including IHC, were investigated in order to determine their usefulness as predictive factors of MSI.
Results Among 120 cases of colorectal cancer, MSI was observed in 15 cases (12.5%), including 11 cases of MSI-H and four cases of MSI-low. Patients with MSI were younger, less than 50 years old, had a family history of cancer, Rt. sided colon cancer and/or synchronous multiple colorectal cancer,mucinous histologic type, and serum carcinoembryonic antigen group in the normal range. Results of multivariate analysis showed Bethesda guidelines, Rt. sided and/or synchronous multiple colorectal cancer, and negative expression of IHC for MLH1, which was consistently associated with MSI-H. MSI-H colorectal tumors have met at least one of these three parameters and their sensitivity and specificity were 100% and 72.5%, respectively.
Conclusion Bethesda guidelines, tumor location, and negative expression of MLH1 protein are important parameters for selection of patients with colorectal cancers for MSI testing. MSI testing is recommended for patients showing any of these three parameters.
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참고문헌 (23)
참고문헌 신청
Alexander J / 2001 / Histopathological identification of colon cancer with microsatellite instability / Am J Pathol 158 : 527 ~ 535
Carethers JM / 1999 / Mismatch repair proficiency and in vitro response to 5-fluorouracil / Gastroenterology 117 : 123 ~ 131
Dietmaier W / 2001 / Detection of microsatellite instability by real time PCR and hybridization probe melting point analysis / Lab Invest 81 : 1453 ~ 1456
Horii A / 1994 / Frequent replication errors at microsatellite loci in tumors of patients with multiple primary cancers / Cancer Res 54 : 3373 ~ 3375
Karnes WE Jr / 1998 / Reduced COX-2 protein in colorectal cancer with defective mismatch repair / Cancer Res 58 : 5473 ~ 5477
Carethers JM / 1999 / Mismatch repair proficiency and in vitro response to 5-fluorouracil / Gastroenterology 117 : 123 ~ 131
Dietmaier W / 2001 / Detection of microsatellite instability by real time PCR and hybridization probe melting point analysis / Lab Invest 81 : 1453 ~ 1456
Horii A / 1994 / Frequent replication errors at microsatellite loci in tumors of patients with multiple primary cancers / Cancer Res 54 : 3373 ~ 3375
Karnes WE Jr / 1998 / Reduced COX-2 protein in colorectal cancer with defective mismatch repair / Cancer Res 58 : 5473 ~ 5477
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