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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 334 - 342 (9page)
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Purpose This preclinical study is to determine whether the capacity of histone deacetylase (HDAC) inhibitors to enhance radiation response depends on temporal sequences of HDAC inhibition and irradiation.
Materials and Methods The effects of HDAC inhibitors trichostatin A (TSA) and SK-7041 on radiosensitivity in human lung cancer cells were examined using a clonogenic assay, exposing cells to HDAC inhibitors in various sequences of HDAC inhibition and radiation. We performed Western blot of acetylated histone H3 and flow cytometry to analyze cell cycle phase distribution.
Results TSA and SK-7041 augmented radiation cell lethality in an exposure time-dependent manner when delivered before irradiation. The impact of TSA and SK-7041 on radiosensitivity rapidly diminished when HDAC inhibition was delayed after irradiation.
Radiation induced the acetylation of histone H3 in cells exposed to TSA, while irradiation alone had no effect on the expression of acetylated histone H3 in TSA-naïve cells. Preirradiation exposure to TSA abrogated radiation-induced G2/M-phase arrest.
When delivered after irradiation, TSA had no effect on the peak of radiation-induced G2/M-phase arrest.
Conclusion TSA and SK-7041 enhances radiosensitivity only when delivered before irradiation.
Unless proven otherwise, it seems prudent to apply scheduling including preirradiation HDAC inhibition so that maximal radiosensitization is obtained.
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참고문헌 (24)
참고문헌 신청
[학술지
Adimoolam S / 2007 / HDAC inhibitor PCI-24781 decreases RAD51 expression and inhibits homologous recombination / Proc Natl Acad Sci U S A 104 : 19482 ~ 19487
Bernhard EJ / 1995 / Effects of ionizing radiation on cell cycle progression. A review / Radiat Environ Biophys 34 : 79 ~ 83
Camphausen K / 2004 / Enhanced radiation-induced cell killing and prolongation of gammaH2AX foci expression by the histone deacetylase inhibitor MS-275 / Cancer Res 64 : 316
Camphausen K / 2005 / Enhancement of in vitro and in vivo tumor cell radiosensitivity by valproic acid / Int J Cancer 114 : 380 ~ 386
Adimoolam S / 2007 / HDAC inhibitor PCI-24781 decreases RAD51 expression and inhibits homologous recombination / Proc Natl Acad Sci U S A 104 : 19482 ~ 19487
Bernhard EJ / 1995 / Effects of ionizing radiation on cell cycle progression. A review / Radiat Environ Biophys 34 : 79 ~ 83
Camphausen K / 2004 / Enhanced radiation-induced cell killing and prolongation of gammaH2AX foci expression by the histone deacetylase inhibitor MS-275 / Cancer Res 64 : 316
Camphausen K / 2005 / Enhancement of in vitro and in vivo tumor cell radiosensitivity by valproic acid / Int J Cancer 114 : 380 ~ 386
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