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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 164 - 170 (7page)
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초록· 키워드
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Purpose :
The purpose of the present study was to assess the biological effects of TNF-alpha in Caco-2 well-differentiated colon adenocarcinoma cells and to determine radiation sensitivity in order to develop TNF-alpha into a cancer therapeutic agent.
Materials and Methods :
A cell viability test was conducted via a colorimetric and colony forming assay after 1 day and 3 days of incubation with TNF-alpha. Western blotting analysis and immunofluorescence staining were conducted to explore TNF-alpha-induced morphological and molecular changes in the adhesion molecules, E-cadherin and claudin-4. The effects of γ-irradiation at a dose of 2 Gy on cell survival were evaluated by a clonogenic assay. The molecular changes in apoptosis-regulatory proteins were assessed by Western blotting.
Results :
Caco-2 cells were highly resistant to TNF alpha-induced cell death and 2 Gy of γ-irradiation. However, we observed the downregulation of the adherens junctional protein, E-cadherin and translocation of tight junctional protein, claudin-4 from the membrane to the cytosol induced by TNF-alpha treatment which would indicate cell-cell junction disruptions. These alterations of junctional proteins influenced the regulation of cell death in response to 2 Gy of γ-irradiation. The combined treatment of TNF-alpha with 2 Gy of γ-irradiation reduced the survival of Caco-2 cells by down-regulating bcl-xl and activating JNK pathways.
Conclusion :
These results suggest that TNF-alpha might be potentially applied as a therapeutic agent in order to enhance sensitivity to 2 Gy of γ-irradiation administered in radiotherapy for the treatment of human colon cancer.
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등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Anderson GM / 2004 / Tumor necrosis factor-alpha in the pathogenesis and treatment of cancer / Curr Opin Pharmacol 4 : 314 ~ 320
Angelini DJ / 2006 / TNF-alpha increases tyrosine phosphorylation of vascular endothelial cadherin and opens the paracellular pathway through fyn activation in human lung en
Bove K / 2001 / Role of ecNOS-derived NO in mediating TNF-induced endothelial barrier dysfunction / Am J Physiol Lung Cell Mol Physiol 280 : L914 ~ 922
Eggermont AM / 2003 / Current uses of isolated limb perfusion in the clinic and a model system for new strategies / Lancet Oncol 4 : 429 ~ 437
Ferreira P / 2005 / Loss of functional E-cadherin renders cells more resistant to the apoptotic agent taxol in vitro / Exp Cell Res 310 : 99 ~ 104
Angelini DJ / 2006 / TNF-alpha increases tyrosine phosphorylation of vascular endothelial cadherin and opens the paracellular pathway through fyn activation in human lung en
Bove K / 2001 / Role of ecNOS-derived NO in mediating TNF-induced endothelial barrier dysfunction / Am J Physiol Lung Cell Mol Physiol 280 : L914 ~ 922
Eggermont AM / 2003 / Current uses of isolated limb perfusion in the clinic and a model system for new strategies / Lancet Oncol 4 : 429 ~ 437
Ferreira P / 2005 / Loss of functional E-cadherin renders cells more resistant to the apoptotic agent taxol in vitro / Exp Cell Res 310 : 99 ~ 104
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