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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제48권 제2호
- 발행연도
- 2014.1
- 수록면
- 100 - 107 (8page)
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Background: KRAS is one of commonly mutated genetic “drivers” in non-small cell lung cancers (NSCLCs). Recent studies indicate that patients with KRAS-mutated tumors do not benefit from adjuvant chemotherapy, so there is now a focus on targeting KRAS-mutated NSCLCs. A feasible mutation detection method is required in order to accurately test for KRAS status. Methods: We compared direct Sanger sequencing and the peptide nucleic acid (PNA)-mediated polymerase chain reaction (PCR) clamping method in 134 NSCLCs and explored associations with clinicopathological factors. Next-generation sequencing (NGS) was used to validate the results of discordant cases. To increase the resolution of low-level somatic mutant molecules, PNA-mediated PCR clamping was used for mutant enrichment prior to NGS. Results: Twenty-one (15.7%) cases were found to have the KRAS mutations using direct sequencing, with two additional cases by the PNA-mediated PCR clamping method. The frequencies of KRAS mutant alleles were 2% and 4%, respectively, using conventional NGS, increasing up to 90% and 89%, using mutant-enriched NGS. The KRAS mutation occurs more frequently in the tumors of smokers (p=.012) and in stage IV tumors (p=.032). Conclusions: Direct sequencing can accurately detect mutations, but, it is not always possible to obtain a tumor sample with sufficient volume. The PNA-mediated PCR clamping can rapidly provide results with sufficient sensitivity.
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참고문헌 (30)
참고문헌 신청
Kosaka T / 2009 / Prognostic implication of EGFR, KRAS, and TP53 gene mutations in a large cohort of Japanese patients with surgically treated lung adenocarcinoma / J Thorac Oncol 4:22~29
Ragusa M / 2013 / Impact of epidermal growth factor receptor and KRAS mutations on clinical outcome in resected non-small cell lung cancer patients / Am J Clin Oncol
Sonobe M / 2012 / Impact of KRAS and EGFR gene mutations on recurrence and survival in patients with surgically resected lung adenocarcinomas / Ann Surg Oncol 19(Suppl 3):S347~S354
Mascaux C / 2005 / The role of RAS oncogene in survival of patients with lung cancer: a systematic review of the literature with meta-analysis / Br J Cancer 92:131~139
Marchetti A / 2009 / Clinical implications of KRAS mutations in lung cancer patients treated with tyrosine kinase inhibitors: an important role for mutations in minor clones / Neoplasia 11:1084~1092
Ragusa M / 2013 / Impact of epidermal growth factor receptor and KRAS mutations on clinical outcome in resected non-small cell lung cancer patients / Am J Clin Oncol
Sonobe M / 2012 / Impact of KRAS and EGFR gene mutations on recurrence and survival in patients with surgically resected lung adenocarcinomas / Ann Surg Oncol 19(Suppl 3):S347~S354
Mascaux C / 2005 / The role of RAS oncogene in survival of patients with lung cancer: a systematic review of the literature with meta-analysis / Br J Cancer 92:131~139
Marchetti A / 2009 / Clinical implications of KRAS mutations in lung cancer patients treated with tyrosine kinase inhibitors: an important role for mutations in minor clones / Neoplasia 11:1084~1092
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