지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제47권 제1호
- 발행연도
- 2013.1
- 수록면
- 52 - 60 (9page)
이용수
초록· 키워드
오류제보하기
Background: The aims of this study were to evaluate the abilities of direct sequencing (DS), peptide nucleic acid (PNA) clamping, and pyrosequencing methods to detect epidermal growth factor receptor (EGFR) mutations in formalin-fixed paraffin-embedded (FFPE) non-small cell lung carcinoma (NSCLC) samples and to correlate EGFR mutational status as determined by each method with the clinical response to EGFR tyrosine kinase inhibitors (TKIs). Methods: Sixty-one NSCLC patients treated with EGFR TKIs were identified to investigate somatic mutations in the EGFR gene (exons 18-21). Results: Mutations in the EGFR gene were detected in 38 of the 61 patients (62%) by DS, 35 (57%) by PNA clamping and 37 (61%) by pyrosequencing. A total of 44 mutations (72%) were found by at least one of the three methods, and the concordances among the results were relatively high (82-85%; kappa coefficient, 0.713 to 0.736). There were 15 discordant cases (25%) among the three different methods. Conclusions: All three EGFR mutation tests had good concordance rates (over 82%) for FFPE samples. These results suggest that if the DNA quality and enrichment of tumor cells are assured, then DS, PNA clamping, and pyrosequencing are appropriate methods for the detection of EGFR mutations.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
[학술지(정기간
Choi JJ / 2009 / Peptide nucleic acid-based array for detecting and genotyping human papillomaviruses / J Clin Microbiol 47 : 1785 ~ 1790
Dufort S / 2011 / Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC / J Exp Clin Cancer Res 30 : 57 ~
Eberhard DA / 2008 / Biomarkers of response to epidermal growth factor receptor inhibitors in Non-Small-Cell Lung Cancer Working Group: standardization for use in the clinica
Goldstraw P / 2007 / The IASLC Lung Cancer Staging Project: proposals for the revision of the TNM stage groupings in the forthcoming (seventh) edition of the TNM Classificat
Choi JJ / 2009 / Peptide nucleic acid-based array for detecting and genotyping human papillomaviruses / J Clin Microbiol 47 : 1785 ~ 1790
Dufort S / 2011 / Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC / J Exp Clin Cancer Res 30 : 57 ~
Eberhard DA / 2008 / Biomarkers of response to epidermal growth factor receptor inhibitors in Non-Small-Cell Lung Cancer Working Group: standardization for use in the clinica
Goldstraw P / 2007 / The IASLC Lung Cancer Staging Project: proposals for the revision of the TNM stage groupings in the forthcoming (seventh) edition of the TNM Classificat
이 논문의 저자 정보
최근 본 자료
댓글(0)
0