지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Ribonucleic acid (RNA) has potential use in forensic science for the determination of postmortem interval. We report the first study on serial sampling of messenger RNA (mRNA) from surgical specimens to determine if there is a correlation between mRNA quantity and elapsed time. Skin tissues were collected from modified radical mastectomy specimens. After a defined period of time, bisected skin sections were cut and frozen in liquid nitrogen. Serial collection of the specimens was conducted, and frozen sections were obtained from all samples. Quantitative real-time reverse transcriptionpolymerase chain reaction was performed using the extracted RNA to measure the transcriptional activity of 2 selected housekeeping genes. The selected loci were mRNA sequences that exhibited time-dependent quantitative changes in a previous study. We collected 44 samples from 9 different patients, with 3-10 samples collected per patient. The amount of mRNA transcripts present in the serial samples showed a weak time-dependent correlation trend only in some cases. Further studies to evaluate different target mRNA sequences are necessary, as is exploration of additional methods to evaluate mRNA transcript degradation.
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참고문헌 (23)
참고문헌 신청
Anderson S / 2005 / A method for determining the age of a bloodstain / Forensic Sci Int 148 : 37 ~ 45
Barrachina M / 2006 / TaqMan PCR assay in the control of RNA normalization in human post-mortem brain tissue / Neurochem Int 49 : 276 ~ 284
Bauer M / 2003 / Quantification of RNA degradation by semi-quantitative duplex and competitive RTPCR: a possible indicator of the age of bloodstains? / Forensic Sci Int 138 :
Bauer M / 2003 / Quantification of mRNA degradation as possible indicator of postmortem interval-- a pilot study / Leg Med 5 : 220 ~ 227
Bauer M. / 2007 / RNA in forensic science / Forensic Sci Int Genet 1 : 69 ~ 74
Barrachina M / 2006 / TaqMan PCR assay in the control of RNA normalization in human post-mortem brain tissue / Neurochem Int 49 : 276 ~ 284
Bauer M / 2003 / Quantification of RNA degradation by semi-quantitative duplex and competitive RTPCR: a possible indicator of the age of bloodstains? / Forensic Sci Int 138 :
Bauer M / 2003 / Quantification of mRNA degradation as possible indicator of postmortem interval-- a pilot study / Leg Med 5 : 220 ~ 227
Bauer M. / 2007 / RNA in forensic science / Forensic Sci Int Genet 1 : 69 ~ 74
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