지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제46권 제1호
- 발행연도
- 2012.1
- 수록면
- 61 - 67 (7page)
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Background: Papillary thyroid carcinoma (PTC) of the thyroid is the most common endocrine malignancy. High prevalence of an activating point mutation of BRAF gene, BRAFV600E, has been reported in PTC. We assessed the efficiency of peptide nucleic acid clamp real-time polymerase chain reaction (PNAcqPCR) for the detection of BRAFV600E mutation in PTC in comparison with direct sequencing (DS). Methods: A total of 265 thyroid lesions including 200 PTCs, 5 follicular carcinomas, 60 benign lesions and 10 normal thyroid tissues were tested for BRAFV600E mutation by PNAcqPCR and DS. Results: The sensitivity and accuracy of the PNAcqPCR method were both higher than those of DS for the detection of the BRAFV600E mutation. In clinical samples, 89% of PTCs harbored the BRAFV600E mutation, whereas 5 follicular carcinomas, 50 benign lesions and 10 normal thyroid tissues lacked the mutation. The mutation was associated with aggressive clinical behaviors as extrathyroid invasion (p=0.015), lymph node metastasis (p=0.002) and multiple tumor numbers (p=0.016) with statistical significance. Conclusions: The PNAcqPCR method is efficiently applicable for the detection of the BRAFV600E mutation in PTCs in a clinical setting.
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참고문헌 (24)
참고문헌 신청
[학술지(정기간행물)] -
Beck TW / 1987 / The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus / Nucleic Acids Res 15 : 595 ~ 609
Bonner TI / 1985 / Structure and biological activity of human homologs of the raf/mil oncogene / Mol Cell Biol 5 : 1400 ~ 1407
Cohen Y / 2003 / BRAF mutation in papillary thyroid carcinoma / J Natl Cancer Inst 95 : 625 ~ 627
Demers DB / 1995 / Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA) / Nucleic Acids Res 23 : 3050 ~ 3055
Beck TW / 1987 / The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus / Nucleic Acids Res 15 : 595 ~ 609
Bonner TI / 1985 / Structure and biological activity of human homologs of the raf/mil oncogene / Mol Cell Biol 5 : 1400 ~ 1407
Cohen Y / 2003 / BRAF mutation in papillary thyroid carcinoma / J Natl Cancer Inst 95 : 625 ~ 627
Demers DB / 1995 / Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA) / Nucleic Acids Res 23 : 3050 ~ 3055
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