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학술저널
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대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제45권 제1호
발행연도
2011.1
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21 - 29 (9page)

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Background: O6-methylguanine-DNA methyltransferase (MGMT) gene promoter methylation is currently the most promising predictive marker for the outcome and benefit from temozolomide treatment in patients with glioblastoma, but there is no consensus on the analysis method for assessing the methylation status in the molecular diagnostic field. The objective of this study was to evaluate methylation-specific polymerase chain reaction (MSP) and pyrosequencing methods for assessing MGMT gene promotor methylation of glioblastoma as well as assessing the MGMT protein expression by immunohistochemistry. Methods: Twenty-seven cases of glioblastoma from the archives at the Department of Pathology Konkuk University Hospital were selected. MGMT promoter methylation was evaluated by MSP and the pyrosequencing methods. The MGMT expression was also measured at the protein level by immunohistochemistry. Results: Overall, MGMT hypermethylation was observed in 44.4% (12/27 cases) of the case of glioblastoma using either MSP or pyrosequencing. The concordant rate was 70.3% (19/27 cases) between MSP and pyrosequencing for MGMT methylation. There was no correlation between MGMT methylation and the protein expression. No significant differences in progression free survival and overall survival were seen between the methylated group and the unmethylated group by using either MSP or pyrosequencing. The status of the MGMT protein expression was correlated with progression free survival (p=0.026). Conclusions: In this study the concordance rate between MSP and the pyrosequencing methods for assessing MGMT gene promotor methylation was relatively low for the cases of glioblastoma. This suggests that more reliable techniques for routine MGMT methylation study of glioblastoma remain to be developed because of quality control and assurance issues.
#Glioblastoma #MGMT protein #human #Pyrosequencing #Methylation-specific polymerase chain reaction #Temozolomide

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참고문헌 (21)

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/ 200911 / The correlation and prognostic significance of MGMT promoter methylation and MGMT protein in glioblastomas / NEUROSURGERY 65 (5) : 866 ~ 875 google schola Brandes AA / 2008 / MGMT promoter methylation status can predict the incidence and outcome of pseudoprogression after concomitant radiochemotherapy in newly diagnosed gliobl google schola Chang-Hyun Lee / 2010 / Epidemiology of Primary Brain and Central Nervous System Tumors in Korea / 대한신경외과학회지 48 (2) : 145 ~ 152 google schola Christmann M / 2010 / MGMT activity, promoter methylation and immunohistochemistry of pretreatment and recurrent malignant gliomas: a comparative study on astrocytoma and gl google schola Costello JF / 1994 / Graded methylation in the promoter and body of the O6-methylguanine DNA methyltransferase (MGMT) gene correlates with MGMT expression in human glioma cel google schola

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