지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Human mitochondrial DNA (mtDNA) is generally used to identify highly degraded forensic samples, particularly when the extracted DNA is not sufficient for nuclear DNA analysis. However, direct sequencing, the most widely used mtDNA analysis method, is laborious and time-consuming, and precludes the simultaneous analysis of many samples. Here, we describe a rapid and simple screening method for mtDNA analysis in Koreans using single base extension (SBE) methods. Sixteen highly polymorphic mtDNA SNPs from the control region were selected, and a multiplex SBE system was constructed to analyze them. Because the developed system consists of two duplex PCRs, which produce small amplicons with fewer than 270 bp, it works well with highly degraded samples such as old skeletal remains. Using this multiplex SBE system, 145 different haplotypes were expected to be observed from 593 unrelated Koreans. Seventy-three haplotypes were expected to be observed only once,and the most frequent haplotype was expected to occur 80 times. Since the mean number of pairwise differences was estimated to be 4.55, the developed system could be useful to exclude samples that do not match evidence and reference samples.
Therefore, the multiplex SBE system used in this study will be a useful tool to analyze many samples simultaneously and to efficiently screen out non-matching mtDNA sequences in forensic casework.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Brandsta ¨tter A / 2006 / Dissection of mitochondrial superhaplogroup H using coding region SNPs / Electrophoresis 27 : 2541 ~ 2550
Butler JM / 2005 / Forensic DNA typing. Biology, technology and genetics of STR markers / Elsevier Academic Press
Butler JM / 2009 / Fundamentals of forensic DNA typing / Elsevier Academic Press
Excoffier L / 2010 / Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows / Mol Ecol Resour 10 : 564 ~ 567
Grignani P / 2009 / Multiplex mtDNA coding region SNP assays for molecular dissection of haplogroups U/K and J/T / Forensic Sci Int Genet 4 : 21 ~ 25
Butler JM / 2005 / Forensic DNA typing. Biology, technology and genetics of STR markers / Elsevier Academic Press
Butler JM / 2009 / Fundamentals of forensic DNA typing / Elsevier Academic Press
Excoffier L / 2010 / Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows / Mol Ecol Resour 10 : 564 ~ 567
Grignani P / 2009 / Multiplex mtDNA coding region SNP assays for molecular dissection of haplogroups U/K and J/T / Forensic Sci Int Genet 4 : 21 ~ 25
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