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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 177 - 183 (7page)
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초록· 키워드
오류제보하기
Human mesenchymal stem cell (hMSCs) isolated from human adult bone marrow have self-renewal capacity and can differentiate into multiple cell types in vitro and in vivo.
A number of studies have now demonstrated that MSCs can differentiate into various neuronal populations. Due to their autologous characteristics, replacement therapy using MSCs is considered to be safe and does not involve immunological complications. The basic helix-loop-helix (bHLH) transcription factor Olig2 is necessary for the specification of both oligodendrocytes and motor neurons during vertebrate embryogenesis. To develop an efficient method for inducing neuronal differentiation from MSCs, we attempted to optimize the culture conditions and combination with Olig2 gene overexpression. We observed neuron-like morphological changes in the hMSCs under these induction conditions and examined neuronal marker expression in these cells by RTPCR and immunocytochemistry. Our data demonstrate that the combination of Olig2 overexpression and neuron-specific conditioned medium facilitates the neuronal differentiation of hMSCs in vitro. These results will advance the development of an efficient stem cell-mediated cell therapy for human neurodegenerative diseases.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Bianco P / 2001 / Bone marrow stromal stem cells: nature, biology, and potential applications / Stem Cells 19 : 180 ~ 192
Boillee S / 2005 / ALS: a disease of motor neurons and their nonneuronal neighbors / Neuron 52 : 39 ~ 59
Lu QR / 2000 / Sonic hedgehog- -regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system / Neuron 25 : 317 ~ 329
Lu QR / 2002 / Common developmental requirement for Olig function indicates a motor neuron/oligodendrocyte connection / Cell 109 : 75 ~ 86
Miller FD / 2007 / Riding the waves: neural and nonneural origins for mesenchymal stem cells / Cell Stem Cell 1 : 129 ~ 130
Boillee S / 2005 / ALS: a disease of motor neurons and their nonneuronal neighbors / Neuron 52 : 39 ~ 59
Lu QR / 2000 / Sonic hedgehog- -regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system / Neuron 25 : 317 ~ 329
Lu QR / 2002 / Common developmental requirement for Olig function indicates a motor neuron/oligodendrocyte connection / Cell 109 : 75 ~ 86
Miller FD / 2007 / Riding the waves: neural and nonneural origins for mesenchymal stem cells / Cell Stem Cell 1 : 129 ~ 130
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