지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 137 - 145 (9page)
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초록· 키워드
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Aggregatibacter actinomycetemcomitans is the most important etiologic agent of aggressive periodontitis and can interact with endothelial cells. Monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) are chemokines, playing important roles in periodontal pathogenesis.
In our current study, the effects of A. actinomycetemcomitans on the production of MCP-1 and IL-8 by human umbilical vein endothelial cells (HUVEC) were investigated. A. actinomycetemcomitans strongly induced the gene expression and protein release of both MCP-1 and IL-8 in a dose- and time-dependent manner. Dead A.
actinomycetemcomitans cells were as effective as live bacteria in this induction. Treatment of HUVEC with cytochalasin D, an inhibitor of endocytosis, did not affect the mRNA up-regulation of MCP-1 and IL-8 by A.
actinomycetemcomitans. However, genistein, an inhibitor of protein tyrosine kinases, substantially inhibited the MCP-1 and IL-8 production by A. actinomycetemcomitans,whereas pharmacological inhibition of each of three members of mitogen-activated protein (MAP) kinase family had little effect. Furthermore, gel shift assays showed that A. actinomycetemcomitans induces a biphasic activation (early at 1-2 h and late at 8-16 h) of nuclear factor-κB (NF-κB) and an early brief activation (0.5-2 h) of activator protein-1 (AP-1). Activation of canonical NF-κB pathway (IκB kinase activation and IκB-α degradation) was also demonstrated in these experiments. Although lipopolysaccharide from A. actinomycetemcomitans also induced NF-κB activation, this activation profile over time differed from that of live A. actinomycetemcomitans. These results suggest that the expression of MCP-1 and IL-8 is potently increased by A. actinomycetemcomitans in endothelial cells,and that the viability of A. actinomycetemcomitans and bacterial internalization are not required for this effect,whereas the activation of protein tyrosine kinase(s), NF-κB,and AP-1 appears to play important roles. The secretion of high levels of MCP-1 and IL-8 resulting from interactions of A. actinomycetemcomitans with endothelial cells may thus contribute to the pathogenesis of aggressive periodontitis.
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참고문헌 (22)
참고문헌 신청
Baker PJ / 2000 / The role of immune responses in bone loss during periodontal disease / Microbes Infect 2 : 1181 ~ 1192
Charo IF / 2006 / The many roles of chemokines and chemokine receptors in inflammation / N Engl J Med 354 : 610 ~ 621
Choi EK / 2005 / Mechanisms of Porphyromonas gingivalis- induced monocyte chemoattractant protein-1 expression in endothelial cells / FEMS Immnol Med Microbiol 44 : 51 ~ 58
Clifton DR / 2005 / Expression and secretion of chemotactic cytokines IL-8 and MCP-1 by human endothelial cells after Rickettsia rickettsii infection: regulation by nuclear t
Dignam JD / 1983 / Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei / Nucleic Acids Res 11 : 1475 ~ 1489
Charo IF / 2006 / The many roles of chemokines and chemokine receptors in inflammation / N Engl J Med 354 : 610 ~ 621
Choi EK / 2005 / Mechanisms of Porphyromonas gingivalis- induced monocyte chemoattractant protein-1 expression in endothelial cells / FEMS Immnol Med Microbiol 44 : 51 ~ 58
Clifton DR / 2005 / Expression and secretion of chemotactic cytokines IL-8 and MCP-1 by human endothelial cells after Rickettsia rickettsii infection: regulation by nuclear t
Dignam JD / 1983 / Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei / Nucleic Acids Res 11 : 1475 ~ 1489
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