지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 173 - 178 (6page)
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초록· 키워드
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Tumor necrosis factor alpha (TNFα) is a multifunctional cytokine that is elevated in inflammatory diseases such as atherosclerosis, diabetes and rheumatoid arthritis. Recent evidence has suggested that β2 adrenergic receptor (β2AR)activation in osteoblasts suppresses osteogenic activity. In the present study, we explored whether TNFα modulates βAR expression in osteoblastic cells and whether this regulation is associated with the inhibition of osteoblast differentiation by TNFα. In the experiments, we used C2C12 cells, MC3T3-E1 cells and primary cultured mouse bone marrow stromal cells. Among the three subtypes of βAR, β2 and β3AR were found in our analysis to be upregulated by TNFα. Moreover,isoproterenol-induced cAMP production was observed to be significantly enhanced in TNFα-primed C2C12 cells, indicating that TNFα enhances β2AR signaling in osteoblasts.
TNFα was further found in C2C12 cells to suppress bone morphogenetic protein 2-induced alkaline phosphatase (ALP) activity and the expression of osteogenic marker genes including Runx2, ALP and osteocalcin. Propranolol, a β2AR antagonist, attenuated this TNFα suppression of osteogenic differentiation. TNFα increased the expression of receptor activator of NF-κB ligand (RANKL), an essential osteoclastogenic factor, in C2C12 cells which was again blocked by propranolol. In summary, our data show that TNFα increases β2AR expression in osteoblasts and that a blockade of β2AR attenuates the suppression of osteogenic differentiation and stimulation of RANKL expression by TNFα. These findings imply that a crosstalk between TNFαand β2AR signaling pathways might occur in osteoblasts to modulate their function.
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참고문헌 (23)
참고문헌 신청
Bonnet N / 2008 / Low dose beta-blocker prevents ovariectomy-induced bone loss in rats without affecting heart functions / J Cell Physiol 217 : 819 ~ 827
Collins S / 1994 / Impaired expression and functional activity of the beta 3- and beta 1-adrenergic receptors in adipose tissue of congenitally obese (C57BL/6J ob/ob) mice /
Cope AP / 1997 / Chronic tumor necrosis factor alters T cell responses by attenuating T cell receptor signaling / J Exp Med 185 : 1573 ~ 1584
El Hadri K / 1996 / Developmental expression and functional activity of beta 1- and beta 3-adrenoceptors in murine 3T3-F442A differentiating adipocytes / Eur J Pharmacol 297
Elefteriou F / 2005 / Leptin regulation of bone resorption by the sympathetic nervous system and CART / Nature 434 : 514 ~ 520
Collins S / 1994 / Impaired expression and functional activity of the beta 3- and beta 1-adrenergic receptors in adipose tissue of congenitally obese (C57BL/6J ob/ob) mice /
Cope AP / 1997 / Chronic tumor necrosis factor alters T cell responses by attenuating T cell receptor signaling / J Exp Med 185 : 1573 ~ 1584
El Hadri K / 1996 / Developmental expression and functional activity of beta 1- and beta 3-adrenoceptors in murine 3T3-F442A differentiating adipocytes / Eur J Pharmacol 297
Elefteriou F / 2005 / Leptin regulation of bone resorption by the sympathetic nervous system and CART / Nature 434 : 514 ~ 520
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