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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 59 - 64 (6page)
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Anthocyanins are naturally occuring phytochemicals and the main components of the coloring of plants, flowers and fruits. They are known to elicit antioxidative, anti-inflammatory and cancer preventive activity. In this study, we investigated anthocyanins in black / yellow soybean seedcoats using different methods of detection - thin layer chromatography (TLC), capillary zone electrophoresis (CZE) and HPLC analysis.
The anthocyanins in soybean seedcoats were extracted by five independent methods of extraction and the aglycons (anthocyanidins) of the corresponding anthocyanins were prepared by acid mediated hydrolysis. The anthocyanin /anthocyanidin in black soybean seedcoat showed characteristic TLC mobility, CZE electrophoretic retention and HPLC migration time while little of anthocyanins were detected from yellow soybean seedcoat. The extracted anthocyanins showed pH dependent retention time in CZE and spectral change in UV-Vis spectrum. HPLC analysis of the hydrolyzed extract of black soybean seedcoat identified the presence of four anthocyanidins. The major anthocyanin in black soybean seedcoat was cyanin (cyanidin-3-O-glucoside),with the relative order of anthocyanidin in cyanidin >delphinidin > petunidin > pelargonidin.
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참고문헌 (19)
참고문헌 신청
Bin Hafeez B / 2008 / Delphinidin, a dietary anthocyanidin in pigmented fruits and vegetables: a new weapon to blunt prostate cancer growth / Cell Cycle 7 : 3320 ~ 3326
Choung MG / 2001 / Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.) / J Agric Food Chem 49 : 5848 ~ 5851
Cvorovic J / 2010 / Oxidative stress-based cytotoxicity of delphinidin and cyanidin in colon cancer cells / Arch Biochem Biophys 501 : 151 ~ 157
Fernandes I / 2010 / Influence of anthocyanins, derivative pigments and other catechol and pyrogallol-type phenolics on breast cancer cell proliferation / J Agric Food Chem 5
Hui C / 2010 / Anticancer activities of an anthocyanin-rich extract from black rice against breast cancer cells in vitro and in vivo / Nutr Cancer 62 : 1128 ~ 1136
Choung MG / 2001 / Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.) / J Agric Food Chem 49 : 5848 ~ 5851
Cvorovic J / 2010 / Oxidative stress-based cytotoxicity of delphinidin and cyanidin in colon cancer cells / Arch Biochem Biophys 501 : 151 ~ 157
Fernandes I / 2010 / Influence of anthocyanins, derivative pigments and other catechol and pyrogallol-type phenolics on breast cancer cell proliferation / J Agric Food Chem 5
Hui C / 2010 / Anticancer activities of an anthocyanin-rich extract from black rice against breast cancer cells in vitro and in vivo / Nutr Cancer 62 : 1128 ~ 1136
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