지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 91 - 101 (11page)
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초록· 키워드
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MspTL is the major surface protein of Treponema lecithinolyticum associated with periodontitis and endodontic infections.
Our recent investigation revealed that MspTL induces proinflammatory cytokines and intercellular adhesion molecule 1 in THP-1 cells and periodontal ligament cells. In this study we conducted oligonucleotide microarray analysis to investigate the global transcriptional regulation in THP-1cells stimulated with purified recombinant MspTL. MspTL upregulated the expression of 90 genes in THP-1 cells at least four fold, and the functions of these genes were categorized into adhesion, apoptosis/antiapoptosis, cell cycle/growth/differentiation,chemotaxis, cytoskeleton organization, immune response, molecular metabolism, proteolysis, signaling, and transcription. The majority of the modified genes are known to be NF-κB-responsive and interferon-stimulated genes (ISGs). The expression of 12 selected genes was confirmed by real-time RT-PCR. Because prostaglandin E2 (PGE2) is an important inflammatory mediator and Cox-2 was found to be induced by MspTL in the microarray analysis, we determined the level of PGE2 in the culture supernatants of MspTL-treated cells and found that MspTL significantly increased PGE2. Our results provide insight into the gene regulation of host cells in response to MspTL, and may contribute to the understanding of the molecular mechanism in periodontitis.
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참고문헌 (20)
참고문헌 신청
Barnes M / 2005 / Experimental comparison and cross-validation of the Affymetrix and Illumina gene expression analysis platforms / Nucleic Acids Res 33 : 5914 ~ 5923
Choi BK / 2001 / Activation of matrix metalloproteinase-2 by a novel oral spirochetal species Treponema lecithinolyticum / J Periodontol 72 : 1594 ~ 1600
Choi BK / 2001 / Effects of whole cell sonicates of Treponema lecithinolyticum on osteoclast differentiation / J Periodontol 72 : 1172 ~ 1177
Choi BK / 2005 / Prostaglandin E2 is a main mediator in receptor activator of nuclear factorkappaB ligand-dependent osteoclastogenesis induced by Porphyromonas gingivalis, Tr
Cohen P / 2000 / Monitoring cellular responses to Listeria monocytogenes with oligonucleotide arrays / J Biol Chem 275 : 11181 ~ 11190
Choi BK / 2001 / Activation of matrix metalloproteinase-2 by a novel oral spirochetal species Treponema lecithinolyticum / J Periodontol 72 : 1594 ~ 1600
Choi BK / 2001 / Effects of whole cell sonicates of Treponema lecithinolyticum on osteoclast differentiation / J Periodontol 72 : 1172 ~ 1177
Choi BK / 2005 / Prostaglandin E2 is a main mediator in receptor activator of nuclear factorkappaB ligand-dependent osteoclastogenesis induced by Porphyromonas gingivalis, Tr
Cohen P / 2000 / Monitoring cellular responses to Listeria monocytogenes with oligonucleotide arrays / J Biol Chem 275 : 11181 ~ 11190
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